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  • Polish Journal Of Microbiology

 

original-paper | 08-September-2020

Characteristics of the Jejunal Microbiota in 35-Day-Old Saba and Landrace Piglets

and coworkers (Fadrosh et al. 2014). Illumina MiSeq PE250 sequencing. The Qubit® 2.0 (Invitrogen, USA) was used to quantify DNA in the samples for library preparation. During the amplification, the barcodes were introduced by the ligated primers, which included sequencing adaptor, barcode, and sequence binding to V3-V4 region. The libraries were sequenced on the MiSeq platform (Illumina, Inc., CA, USA). All jejunal content samples from 18 piglets were subjected to 16S rRNA sequencing; however, one

HUAN GAO, YUTING YANG, ZHENHUI CAO, JINMING RAN, CHUNYONG ZHANG, YING HUANG, MINGHUA YANG, SUMEI ZHAO, QINGCONG AN, HONGBIN PAN

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 367–378

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