original-paper | 08-September-2020
and coworkers (Fadrosh et al. 2014).
Illumina MiSeq PE250 sequencing. The Qubit® 2.0 (Invitrogen, USA) was used to quantify DNA in the samples for library preparation. During the amplification, the barcodes were introduced by the ligated primers, which included sequencing adaptor, barcode, and sequence binding to V3-V4 region. The libraries were sequenced on the MiSeq platform (Illumina, Inc., CA, USA). All jejunal content samples from 18 piglets were subjected to 16S rRNA sequencing; however, one
Polish Journal of Microbiology, Volume 69 , ISSUE 3, 367–378