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Original Paper

Transferrin and Lactoferrin – Human Iron Sources for Enterococci

To overcome limitations in iron acquisition, enterococci have evolved a number of mechanisms to scavenge iron from the host iron-binding proteins – transferrin (TR) and lactoferrin (LF). The aim of this study was to demonstrate the mechanisms by which enterococci utilize human TR and LF bound iron. The study included two strains of Enterococcus faecalis grown in iron-deficient and iron-excess media respectively. The binding activity of both proteins was monitored using proteins labelled

Paweł Lisiecki

Polish Journal of Microbiology , ISSUE 4, 419–425

Short Communication

Antiviral Resistance of Splenocytes in Aged Mice

We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated the antiviral actions of lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse lactoferrin (rmLF) and bovine lactoferrin (bLF) were used. There were no differences in the susceptibility to EMCV infection in the studied age categories. Both types of lactoferrins were protective in young and old mice. The study confirmed the undisturbed

Ewa Zaczyńska, Jolanta Artym, Maja Kocięba, Timo Burster, Marian Kruzel, Maria Paprocka, Michał Zimecki

Polish Journal of Microbiology , ISSUE 1, 131–134

Article

BENEFICIAL EFFECT OF LACTOFERRIN ON THE MICROBIOTA FROM GASTROINTESTINAL TRACT

trawiennymi [51]. Peptydy z LF zwykle działają silniej niż wyjściowe, natywne białko. Na przykład natywna bydlęca LF stymulowała wzrost B. breve w stężeniu 300 μg/ml, a jej pepsynowy hydrolizat już w stężeniu 10 μg/ml. Najsilniej bifidogennie działał niewielki peptyd z końca N cząsteczki złożony z dwóch fragmentów 16- i 6-aminokwasowego połączonych mostkiem disiarczkowym i nazwany przez autorów bifidogennym peptydem laktoferynowym (bifidogenic lactoferrin peptide; BLP). Jak sugerują badacze, aktywność

Jolanta Artym, Michał Zimecki

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 3, 277–290

Short Communication

Qualitative and Quantitative Characteristics of Selected Bacterial Groups in Children with Inflammatory Bowel Diseases

The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selected microorganisms depends on the level of clinical activity of disease and is correlated with faecal concentration of inflammatory markers. Differences in microflora disturbance

Dorota Roszak, Mirosława Gałęcka, Wojciech Cichy, Patrycja Szachta

Polish Journal of Microbiology , ISSUE 2, 219–224

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