Case report | 01-December-2019
type 4.2.2, in 1 of 18 with weak D type 11, in 1 of 17 with weak D type 15, and in 1 weak D type 33 individual. Anti-D was demonstrated to be an alloantibody in weak D type 4.0, type 4.2.2, and type 15 individuals, but an autoantibody in weak D type 11 and type 33 individuals. In conclusion, only a complete serologic investigation of individuals with a given weak D type identified by molecular analysis allows concluding on the nature of the antibody. Transfusing weak D type 4.2.2 and type 15
Bach-Nga Pham,
Michèle Roussel,
Dominique Gien,
Maryline Ripaux,
Carine Auxerre,
Pierre-Yves Le Pennec,
Christine Andre-Botte
Immunohematology, Volume 29 , ISSUE 2, 55–62
research-article | 28-April-2020
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–12
Report | 25-March-2020
Kim Hue-Roye,
Sunitha Vege
Immunohematology, Volume 24 , ISSUE 4, 170–175
research-article | 30-November-2019
Joaquín Abolafia,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–20
Article | 03-December-2017
REYES PENA-SANTIAGO,
INGRID VARELA
Journal of Nematology, Volume 49 , ISSUE 3, 245–253
Research Article | 26-September-2018
ELENA FANELLI,
ALBERTO TROCCOLI,
NICOLA VOVLAS,
GIANLUCA SCARCIA,
ANNAMARIA MINCUZZI,
SIMONA M. SANZANI,
ANTONIO IPPOLITO,
FRANCESCA DE LUCA
Journal of Nematology, Volume 49 , ISSUE 4, 418–426
research-article | 19-February-2021
on morphological characteristics and molecular analysis, from eight marine fish species in the South of Vietnamese water and recorded seven fish species as new intermediate hosts of this Anisakis nematode. Genetically, the ITS1-5.8S-ITS2 sequences of Vietnamese A. typica were close to those from Asian countries and Australia, to make a common group separated from another group from America and Europe.
Hoang Van Hien,
Bui Thi Dung,
Ha Duy Ngo,
Pham Ngoc Doanh
Journal of Nematology, Volume 53 , 1–9
research-article | 30-November-2019
Francisco Jorge Carlos Souza Junior,
Mayara Castro Assunção
Journal of Nematology, Volume 52 , 1–4
research-article | 30-November-2018
., 2017) have repeatedly confirmed the monophyly of both Insectivorus- and Dolichura-groups, and most of them agree that these groups are sister groups. Nevertheless, two contributions (Darsouei et al., 2014; Zhou et al., 2017) do not support this idea.
The molecular analysis of 18S and 28S rDNA sequences of the new species herein described, whose results are presented in the trees of Figures 4 and 5, respectively, confirm the monophyly of both species groups as well as that they are sister groups
Joaquín Abolafia,
Reyes Peña-Santiago
journal of nematology, Volume 51 , 1–21
Report | 25-March-2020
Christine Lomas-Francis,
Helene DePalma
Immunohematology, Volume 24 , ISSUE 4, 180–190
Short Communication | 28-June-2017
The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of
Beata Zimowska,
Ewa Dorota Zalewska,
Ewa Dorota Król,
Agnieszka Furmańczyk
Polish Journal of Microbiology, Volume 66 , ISSUE 2, 281–285
research-article | 30-November-2020
) μm and c′ = 2.10 ± 0.33 (1.84-2.52) μm. Total body length was 535.33 ± 21.58 (508.63-562.70) μm, with a = 24.47 ±1.37 (23.36-27.91) μm, b = 7.73 ± 1.01 (6.14-9.50) μm, and b′ = 4.22 ± 0.53 (3.67-5.15) μm.
The sequences of the studied rDNA regions were submitted to GenBank (ITS: MT994745, MW350685-MW350688 and D2-D3 28S: MT994748, MW349659-MW349662). The populations CFN001, CFN003, CFN004, and CFN005 used for molecular analysis showed a high degree of sequence identity (99%) with P. zeae from
Francisco Jorge Carlos Souza Junior,
Mayara Castro Assunção
Journal of Nematology, Volume 53 , 1–5
research-article | 30-November-2019
M. R. Moore,
J. A. Brito,
S. Qiu,
C. G. Roberts,
L. A. Combee
Journal of Nematology, Volume 52 , 1–4
research-article | 30-November-2020
.
The identification of the nematode characterized as Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949, was based on morphological characters of adults, esterase phenotypes (n = 16), and molecular analysis. Morphological identification was performed by analyzing the male labial region, and presented a trapezoidal shape and prominent concave labial disk compared to the submedian lips and transverse striations on the head annule; the stylet basal knobs were higher than wide (n = 12; Fig
R. F. Gonsaga,
A. Souza Pollo,
D. D. Nascimento,
R. J. Ferreira,
L. T. Braz,
P. L. M. Soares
Journal of Nematology, Volume 53 , 1–4
research-article | 30-November-2018
1000X magnification with compound microscope and SEM.
Molecular analysis of the ribosomal RNA and mitochondrial gene sequences of the extracted nematodes confirmed their species as P. vulnus (Table 1). The rDNA LSU region (D2A/D3B: ACAAGTACCGTGAGGGAAAGTTG/TCGGAAGGAACCAGCTACTA) (Nunn, 1992), rDNA ITS region (TW81/AB28: GTTTCCGTAGGTGAACCTGC/ATATGCTTAAGTTCAGCGGGT) (Amiri et al., 2002; Subbotin et al., 2001), rDNA SSU region (SSU18A/SSU26R: AAAGATTAAGCCATGCATG/CATTCTTGGCAAATGCTTTCG) (Eyualem and
Yu-po Lin,
Wan-chun Lee,
Pei-che Chung,
Jiue-in Yang
Journal of Nematology, Volume 51 , 1–5
Case report | 12-March-2020
Antibodies of apparent D specificity may be found in D+ patients. We report a D+, multi-transfused Caucasian woman with myelodysplasia who exhibited several alloantibodies. One antibody was a moderately strong (2+) anti-D that persisted for 9 months, until the woman died. Molecular analysis of the patient’s RHD gene identified the rare weak D type 21 (938C>T) allele. D alloantibodies do not occur in patients with most weak D types, but some patients with a weak D phenotype, including
Heather McGann,
Robert E. Wenk
Immunohematology, Volume 26 , ISSUE 1, 27–29
research-article | 14-December-2020
Jacob, 2012, and Oscheius amsactae Ali, Pervez, Andrabi, Sharma and Verma, 2011 and Metarhabditis longicaudata Tabassum, Salma and Nasir, 2019. Most of these studies, however, have characterized the species morphologically and morphometrically. Regarding molecular analysis, several Internal Transcribed Spacer (ITS) rDNA sequences, obtained from M. amsactae isolated in India, Philippines, and Pakistan have been deposited in the GenBank, but none of the nematode specimens used to obtain the sequences
Aashaq Hussain Bhat,
Shreyansh Srivastava,
Aasha Rana,
Ashok Kumar Chaubey,
Ricardo A. R. Machado,
Joaquín Abolafia
Journal of Nematology, Volume 52 , 1–23
research-article | 30-November-2018
Mehmet Dayi,
Ece B. Kasapoğlu Uludamar,
Süleyman Akbulut,
İ. Halil Elekcioğlu
journal of nematology, Volume 51 , 1–6
research-article | 30-November-2019
M. Clara Vieira dos Santos,
M. Teresa M. Almeida,
Sofia R. Costa
Journal of Nematology, Volume 52 , 1–4
research-article | 06-November-2020
Thi Anh Duong Nguyen,
Reyes Peña-Santiago
Journal of Nematology, Volume 52 , 1–8
research-article | 26-March-2021
The genus Bitylenchus belongs to the family Dolichodoridae Chitwood in Chitwood, 1950. This genus has been synonymized with Tylenchorhynchus (Geraert, 2011). However, Handoo et al. (2014) and Hosseinvand et al. (2020) considered it as a valid taxon using molecular analysis. Siddiqi (2000) considered 29 valid species under the genus Bitylenchus. Members of Bitylenchus and Tylenchorhynchus differ in areolated outer bands of lateral fields, a large postanal intestinal sac containing intestinal
Ebrahim Shokoohi
Journal of Nematology, Volume 53 , 1–9
research-article | 16-April-2019
were deposited at Nematology Collection of the Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. Two paratype females were also deposited at Nematode Collection of the University of Jaen, Spain.
Etymology
The new species is named in honor of Dr. Hassan Barooghi, the late Entomologist and Associate Professor in Department of Plant Protection, University of Tabriz, Tabriz, Iran.
Molecular characterization and phylogeny
For molecular analysis, one D2–D3
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Arezoo Naghavi
Journal of Nematology, Volume 51 , 1–17
Original Paper | 10-December-2018
Abstract
The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis
EULALIA MACHOWICZ-MATEJKO,
AGNIESZKA FURMAŃCZYK,
EWA DOROTA ZALEWSKA
Polish Journal of Microbiology, Volume 67 , ISSUE 4, 407–416
Research Article | 03-September-2018
anus distance); and elongate, conical tail with pointed tip. Nothotylenchus andrassy n. sp. is morphologically similar to five known species of the genus, namely Nothotylenchus geraerti, Nothotylenchus medians, Nothotylenchus affinis, Nothotylenchus buckleyi, and Nothotylenchus persicus. The results of molecular analysis of rRNA gene sequences, including the D2–D3 expansion region of 28S rRNA, internal transcribed spacer (ITS) rRNA and partial 18S rRNA gene are provide for the new species.
Parisa Jalalinasab,
Mohsen Nassaj Hosseini,
Ramin Heydari
Journal of Nematology, Volume 50 , ISSUE 2, 219–228
Article | 21-July-2017
An aberrant specimen of Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae) also known as red palm weevil (RPW), the most economically important insect pest of palms in the world, was found among a batch of conspecifics reared for research purposes. A morphological analysis of this weevil revealed the presence of nematodes associated with a structured cuticle defect of the thorax. These nematodes were not able to be cultured, but were characterized by molecular analysis using 28S
MANUELA CAMEROTA,
GIUSEPPE MAZZA,
LYNN K. CARTA,
FRANCESCO PAOLI,
GIULIA TORRINI,
CLAUDIA BENVENUTI,
BEATRICE CARLETTI,
VALERIA FRANCARDI,
PIO FEDERICO ROVERSI
Journal of Nematology, Volume 48 , ISSUE 1, 1–6
Original Paper | 30-June-2018
morphological and molecular analysis, the isolates were identified as Trichoderma. The most frequently identified strains belonged to Trichoderma harzianum species. Among all strains, the most effective in degradation of cellulose and xylose was T. harzianum and T. virens, especially those isolated from forest wood, forest soil or garden and mushroom compost. The results of this work confirmed that numerous strains from the Trichoderma species have high cellulose and xylan degradation potential and could be
ROMAN MARECIK,
LIDIA BŁASZCZYK,
RÓŻA BIEGAŃSKA-MARECIK,
AGNIESZKA PIOTROWSKA-CYPLIK
Polish Journal of Microbiology, Volume 67 , ISSUE 2, –
research-article | 30-November-2019
Ramandeep K. Sandhi,
Ratnasri Pothula,
Satyendra K. Pothula,
Byron J. Adams,
Gadi V.P. Reddy
Journal of Nematology, Volume 52 , 1–11
research-article | 30-November-2019
-27.3) μm; b 5.0 (4.6-6.0) μm; and V 51.4% (47.5-54.1%) μm. Measurements of morphological characters of adult females were similar to N. minor (Colbran) Siddiqi (Decraemer, 1995; Hajihassani et al., 2018a). Due to similar morphometrics of N. minor and closely related species of Nanidorus, a molecular analysis was first performed to confirm the morphological identification by targeting three different regions of the genomic DNA. Briefly, DNA samples were extracted from single stubby-root nematodes (n
Ganpati B. Jagdale,
Fereidoun Forghani,
Katherine Martin,
Abolfazl Hajihassani,
Alfredo Dick Martinez-Espinoza
Journal of Nematology, Volume 52 , 1–3
Article | 16-February-2021
M.R. George
Immunohematology, Volume 35 , ISSUE 4, 154–155
Article | 21-July-2017
–30 mm). It is distinguished from H. elachista by larger cyst (513.4–778.3 mm3 343.4–567.1 mm vs. 350–560 mm 3 250–450 mm) and longer stylet length of J2 (23.8–25.3 mm vs. 17–19 mm). Molecular analysis of rRNA large subunit (LSU) D2–D3 segments and ITS gene sequence shows that H. sojae n. sp. is more close to rice cyst nematode H. elachista than H. glycines. Heterodera sojae n. sp. was widely distributed in Korea. It was found from soybean
HEONIL KANG,
GEUN EUN,
JIHYE HA,
YONGCHUL KIM,
NAMSOOK PARK,
DONGGEUN KIM,
INSOO CHOI
Journal of Nematology, Volume 48 , ISSUE 4, 280–289
research-article | 30-November-2020
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Arezoo Naghavi,
Reyes Peña-Santiago
Journal of Nematology, Volume 53 , 1–12
research-article | 30-March-2020
Andrea M. Skantar,
Zafar A. Handoo,
Mihail R. Kantor,
Lynn K. Carta,
Jamal Faghihi,
Virginia Ferris
Journal of Nematology, Volume 52 , 1–8
research-article | 30-November-2019
), body width (20.0, 15.0-28.5), head end to posterior end of esophageal glands (104.0, 98.0-111.0), anal body width (12.0, 10.5-13.0), tail length (26.0, 21.0-29.0), a (25.0, 20.0-32.0), b (4.2, 3.6-4.7), c (17.0, 15.0-21.0), c’ (2.1, 1.6-2.5) and V (77.0%, 74.0-79.0%). Four lines are present in the lateral field.
The morphometric details of females were recorded and compared to closely related species which were consistent with Pratylenchus hippeastri (Inserra et al., 2007).
Molecular analysis
Zafar A. Handoo,
Andrea M. Skantar,
Mihail R. Kantor,
Saad L. Hafez,
Maria N. Hult
Journal of Nematology, Volume 52 , 1–5
research-article | 30-November-2018
Ruihang Cai,
Antonio Archidona-Yuste,
Carolina Cantalapiedra-Navarrete,
Juan E. Palomares-Rius,
Jingwu Zheng,
Pablo Castillo
Journal of Nematology, Volume 51 , 1–21
Article | 10-April-2021
recommended in 20152 to phase-in RHD genotyping for patients with a serologic weak D phenotype. The same authors3 specified their recommendation in 2020 to phase-out the reporting of a “serologic weak D phenotype” and resolve all weak D types with RHD genotyping.
We report a pregnant woman with anti-U and a serologic weak D phenotype. The clinical workup in this case illustrated the importance of molecular analysis of serologic weak D phenotypes early in the pregnancy to preserve rare D– RBC units and to
Q. Yin,
K. Srivastava,
D.G. Brust,
W.A. Flegel
Immunohematology, Volume 37 , ISSUE 1, 1–4
Original Paper | 30-June-2018
production, bacterial growth rate in milk as well as liberation of biologically active peptides. The study reveals significant differences in the presence of various variants of CEPs encoding genes among ten novel Polish strains and indicates the intraspecific diversity exhibited by L. helveticus. In terms of distribution of CEPs genes, four different genetic profiles were found among the microorganisms analyzed. Furthermore, the strains exhibited also various levels of proteolytic activity. Molecular
KATARZYNA W. SKRZYPCZAK,
WALDEMAR Z. GUSTAW,
ADAM D. WAŚKO
Polish Journal of Microbiology, Volume 67 , ISSUE 2, 203–211
original-paper | 05-August-2020
personal status, residency, and source of water supply. The collected fresh stool samples were microscopically examined using the iodine and saline wet mount microscopy to detect Entamoeba trophozoites and/or cysts. About 0.2 g of each specimen was preserved at –80°C for molecular analysis.
DNA was extracted from specimens using the QiaAmp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany) following the manufacturer protocol. Finally, the purified DNA concentrate was eluted from the silica membrane spin
SHLER AKRAM FAQE MAHMOOD,
HAWRI MUSTAFA BAKR
Polish Journal of Microbiology, Volume 69 , ISSUE 3, 263–272
Research Article | 03-September-2018
molecular analysis of many D. weischeri specimens from Canada is presented. Individuals from 41C. arvense or yellow pea grain samples with seeds of C. arvense from the Prairie Provinces were sequenced for the internal transcribed spacer (ITS rDNA), large subunit (LSU) D2D3 28S rDNA, partial segment of small subunit (SSU) 18S rDNA, and the heat shock protein Hsp90 gene. The analysis also included D. weischeri individuals from C. arvense from Russia and garlic with D. dipsaci from the Provinces of Ontario
Mehrdad Madani,
Mario Tenuta
Journal of Nematology, Volume 50 , ISSUE 2, 163–182
research-article | 30-November-2018
) and observed under a scanning electron microscope (Jeol JSM-6390) at 10 kV.
Molecular study
For molecular analysis, DNA was extracted with DNeasy Blood and Tissue Kit (Qiagen Inc., Valencia, CA). Two rRNA fragments, i.e. the LSU D2–D3 and ITS regions, were amplified. Primers for D2–D3 segments amplification were D2A (5′-ACAAGTACCGTGAGGGAAAGTTG-3′) and D3B (5′-TCGGAAGGAACCAGCTACTA-3′). Primers for ITS amplification were TW81 (5′-GTTTCCGTAGGTGAACCTGC-3′) and AB28 (5′-ATATGCTTAAGTTCAGCGGGT-3
Heonil Kang,
Jongmin Seo,
Garam Han,
Donggeun Kim,
Insoo Choi
Journal of Nematology, Volume 51 , 1–12
Article | 24-July-2017
National collection of Insects, Arachnids, and Nematodes (Accession no. 14851 to 14853 for the second stage juveniles and 14854–14855 for the cyst cones). For molecular analysis,DNA was extracted from individual juvenile (n = 4) from different cysts. A 1,151-bp fragment of ribosomal DNA containing ITS1-5.8SITS2 region was amplified and sequenced using primers 18S (5'-TTGATTACGTCCCTGCCCTTT-3') and 26S (5'- TTTCACTCGCCGTTACTAAGG-3') (Vrain et al., 1992). The sequence was
FENGCHENG SUN,
NEIL HENRY,
QING YU
Journal of Nematology, Volume 49 , ISSUE 2, 131–132