Article
A population of Xiphinema chambersi from the root zone around live oak (Quercus virginiana Mill.) trees on Jekyll Island, GA, is described using both morphological and molecular tools and compared with descriptions of type specimens. Initially, because of a few morphological differences, this nematode was thought to represent an undescribed species. However, on further examination, the morphometrics of the nematodes from live oak tend to agree with most of the morphometrics in the
ZAFAR A. HANDOO,
LYNN K. CARTA,
ANDREA M. SKANTAR,
SERGEI A. SUBBOTIN,
STEPHEN W. FRAEDRICH
Journal of Nematology , ISSUE 1, 20–27
research-article
measurements are provided in Table 1.
Table 1.
Morphometrics of female of Deladenus gilanica n. sp. from Iran.
Mycetophagous stage
Infective stage
Characters
Holotype female
Paratype females
Paratype females
n
−
10
4
L
367
364.8 ± 33.7 (314-422)
330.3 ± 17.6 (310-341)
L'
361
335.4 ± 33.3 (285-393)
294.7 ± 15.3 (277-304)
a
18.6
21.1 ± 2.6 (18.1-26.5)
28.3 ± 0.1 (28.2-28.4)
b
8.5
7.1 ± 0.9 (5.9-8.5)
6.2 ± 1.8 (5.1-8.3)
b'
5.7
5.0 ± 0.9 (4.1-6.9)
3.7 ± 0.4 (3.4-4.1)
c
13.4
Parisa Jalalinasab,
Mehrab Esmaeili,
Weimin Ye,
Ramin Heydari
Journal of Nematology , 1–10
Research Article
During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization
Ilia Mariana Escobar-Avila,
Edgar Óliver López-Villegas,
Sergei A. Subbotin,
Alejandro Tovar-Soto
Journal of Nematology , ISSUE 2, 229–242
research-article
convergence, the remaining samples were retained for further analyses. The topologies were used to generate a 50% majority rule consensus tree. Posterior probabilities (PP) are given on appropriate clades. Trees from all analyses were visualized using FigTree software V.1.42 (http://tree.bio.ed.ac.uk/software/figtree/).
Results
Systematics
Xiphinema histriae Lamberti et al. (1993a, 1993b).
(Figs. 1–3; Table 2).
Table 2.
Comparative morphometrics of females and males of Xiphinema histriae (Lamberti
Ruihang Cai,
Antonio Archidona-Yuste,
Carolina Cantalapiedra-Navarrete,
Juan E. Palomares-Rius,
Jingwu Zheng,
Pablo Castillo
Journal of Nematology , 1–21
Research Article
morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus, agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus, meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences
Farzad Aliramaji,
Ebrahim Pourjam,
Sergio Álvarez-Ortega,
Farahnaz Jahanshahi Afshar,
Majid Pedram
Journal of Nematology , ISSUE 3, 437–452
Article
Plant-parasitic nematodes such as Longidorus euonymus and Helicotylenchus multicintctus are species widely distributed in central Europe as well as in Mediterranean area. In Greece, both species have been previously reported but no morphometrics or molecular data were available for these species. Nematode surveys in the rhizosphere of grapevines in Athens carried out in 2016 and 2017, yielded a Longidorus species identified as Longidorus euonymus. Similarly, a population of Helicotylenchus
EMMANUEL A. TZORTZAKAKIS,
CAROLINA CANTALAPIEDRA-NAVARRETE,
PABLO CASTILLO,
JUAN E. PALOMARES-RIUS,
ANTONIO ARCHIDONA-YUSTE
Journal of Nematology , ISSUE 3, 233–235
research-article
overlapping morphometrics of some species groups in the genus Xiphinema make quarantine regulations and protection methods more difficult. Therefore, accurate identification of Xiphinema species using integrate approach is strongly recommended to create a basis for plant pest management.
In Vietnam, eight species of the genus Xiphinema have been reported, however, molecular identification are not available for most of them (Nguyen and Nguyen, 2000), and thus, a higher diversity of Xiphinema spp. is
Huu Tien Nguyen,
Thi Duyen Nguyen,
Thi Mai Linh Le,
Quang Phap Trinh
Journal of Nematology , 1–4
Research Article
Munawar Maria,
Weimin Ye,
Qing Yu,
Jianfeng Gu
Journal of Nematology , ISSUE 3, 369–386
Original Research
Abstract Pseudacrobeles (Pseudacrobeles) curvatus sp. n. was collected from potato fields in Gyeongsangnam-do, South Korea. The new species shares morphological characters typical of the genus Pseudacrobeles, including three lateral incisures that fade posteriorly near the phasmid openings. The new species differs from other Pseudacrobeles species by its smaller body size and a comparatively shorter corpus relative to the isthmus length. In this study, we provide a comparison of morphometrics
Jiyeon Kim,
Taeho Kim,
Joong-Ki Park
Journal of Nematology , ISSUE 2, 162–167
Original Research
Qing Yu,
Jianfeng Gu,
Weimin Ye,
Rusong Li,
Jie He
Journal of Nematology , ISSUE 2, 168–176
research-article
M. Clara Vieira dos Santos,
M. Teresa M. Almeida,
Sofia R. Costa
Journal of Nematology , 1–4
research-article
The genus Pungentus is an interesting dorylaimid genus, often found in forest habitats of the Northern Hemisphere, and with very restricted presence in southern territories. Its taxonomy was updated by Álvarez-Ortega and Peña-Santiago (2014), who listed 16 valid species and other four inquirendae or incertae sedis and provided a key to their identification as well a compendium of their main morphometrics.
Available information about Pungentus species from Iran is very limited. Solouki et al
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Reyes Peña-Santiago
Journal of Nematology , 1–12
research-article
. In the present study, a Georgian population of P. strongyloides recovered for the first time from L. ibericus and was fully characterized by morphology and morphometrics. Furthermore, the PCR amplification and sequencing of the D2 to D3 expansion domains of the 28S rRNA gene, the ITS, and the mitochondrial COI were carried out. The phylogenetic relationships of P. strongyloides from Georgia to other Pelodera species and Rhabditidae were also reconstructed.
Materials and methods
Nematode
O. Gorgadze,
A. Troccoli,
E. Fanelli,
E. Tarasco,
F. De Luca
Journal of Nematology , 1–12
Article
molecular data support a relationship with Aporcelaimellus, which, however, might not be as close as usually assumed. An emended diagnosis of the genus, a key to species identification, and a compendium of their morphometrics are provided. Makatinus siddiqii is regarded as species inquirenda, Makatinus simus is retained under Eudorylaimus, and Makatinus tritici becomes a junior synonym of Aporcelaimellus tritici.
REYES PENA-SANTIAGO,
INGRID VARELA
Journal of Nematology , ISSUE 3, 245–253
research-article
warts of the lateral fields); (G, H) Tail in ventral and lateral views, respectively (arrow at phasmid); (I) Lateral field.
Material examined
Fifty one females in generally acceptable state of preservation.
Measurements
See Table 1.
Table 1.
Morphometrics of Oscheius saproxylicus sp. n. Measurements in μm and in the form: mean ± standard deviation (range) where appropriate.
Locality
Puente de la Sierra
Province
Jaén
Habitat
Dead wood
n
Holotype female
Paratypes 50 females
Joaquín Abolafia,
Reyes Peña-Santiago
journal of nematology , 1–21
Article
and 18S ribosomal DNA and shown to belong to the family Panagrolaimidae (Rhabditida), within a clade of Panagrellus. While most nematodes in the insect were juveniles, a single male adult was partially characterized by light microscopy. Morphometrics showed similarities to a species described from Germany. Excluding the entomopathogenic nematodes (EPN), only five other genera of entomophilic or saprophytic rhabditid nematodes are associated with this weevil. This is the first
MANUELA CAMEROTA,
GIUSEPPE MAZZA,
LYNN K. CARTA,
FRANCESCO PAOLI,
GIULIA TORRINI,
CLAUDIA BENVENUTI,
BEATRICE CARLETTI,
VALERIA FRANCARDI,
PIO FEDERICO ROVERSI
Journal of Nematology , ISSUE 1, 1–6
Research Article
juvenile Oscheius rugaoensis (Zhang et al., 2012) Darsouei et al., 2014 (Rhabditidae), and juvenile and adult Mononchoides sp. (Diplogastridae) based on images, morphometrics, and sequences of 18S and 28S rDNA. A novel short 28S sequence of a separate population of Oscheius necromenus SB218 from Australian millipedes was also included in a phylogenetic comparison of what can now be characterized as a species complex of millipede-associated Oscheius. The only other nematode associates of millipedes
L. K. Carta,
W. K. Thomas,
V. B. Meyer-Rochow
Journal of Nematology , ISSUE 4, 479–486
Article
MEHRAB ESMAEILI,
RAMIN HEYDARI,
MOZHGAN ZIAIE,
JIANFENG GU
Journal of Nematology , ISSUE 1, 34–42
Research Article
. fukushimae on Japanese millipedes. In addition, the authors found three new Pristionchus species on millipedes, which are described as P. riukiariae sp. n., P. degawai sp. n., and P. laevicollis, sp. n., the latter of which was also found on stag beetles. These species are most closely related to P. maxplancki, P. japonicus, and P. quartusdecimus and belong to the pacificus species-complex. The authors describe all species based on morphology, morphometrics, and genome-wide sequence analysis. Mating
Natsumi Kanzaki,
Matthias Herrmann,
Kohta Yoshida,
Christian Weiler,
Christian Rödelsperger,
Ralf J. Sommer
Journal of Nematology , ISSUE 4, 587–610
Research Article
Munawar Maria,
Ruihang Cai,
Weimin Ye,
Thomas O. Powers,
Jingwu Zheng
Journal of Nematology , ISSUE 4, 611–622
Article
inner incisures weakly crenated, excretory pore situated 90 to 100 mmfrom anterior end; functional males common in the population, with spicules 24 to 26 mmlong. Tail of both sexes similar, almost straight and elongate-conoid. The new species resembles in morphology and morphometrics to four known species of the genus, namely P. apitica, P. minuta, P. myceliophaga, and P. sohailai. The results of phylogenetic analyses based on sequences of D2/D3 expansion region of 28S rRNA gene
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology , ISSUE 2, 109–115
Research Article
Two different nematode species were recovered from pomegranate decaying fruit in two localities in Southern Italy: the mycetophagus nematode Sheraphelenchus sucus and a bacterial feeder nematode belonging to the Panagrolaimidae (Rhabditida) family. Morphometrics of the Italian population of S. sucus closely resemble that of the type population, whereas some differences were found when compared with another population from Iran. Molecular characterization of the Italian S. sucus using the 18S
ELENA FANELLI,
ALBERTO TROCCOLI,
NICOLA VOVLAS,
GIANLUCA SCARCIA,
ANNAMARIA MINCUZZI,
SIMONA M. SANZANI,
ANTONIO IPPOLITO,
FRANCESCA DE LUCA
Journal of Nematology , ISSUE 4, 418–426
research-article
sister group to all described beetle-associated Pristionchus species. Thus, the gonochorist P. paulseni sp. n. has a crucial position in the phylogeny of Pristionchus. In order to characterize both species, morphology, morphometrics, mating experiments and genome-wide transcriptome sequencing are used.
Materials and methods
Nematode cultivation
One strain of P. paulseni sp. n. was isolated from an adult of Lucanus dybowski taiwanus (Miwa, 1937) (Coleoptera: Lucanidae) collected at Pilu sacred tree
Matthias Herrmann,
Natsumi Kanzaki,
Christian Weiler,
Kohta Yoshida,
Christian RÖdelsperger,
Ralf J. Sommer
Journal of Nematology , 1–14
Article
SERGIO ALVAREZ-ORTEGA,
THI ANH DUONG NGUYEN,
JOAQUI´N ABOLAFIA,
MICHAEL BONKOWSKI,
REYES PEN˜A-SANTIAGO
Journal of Nematology , ISSUE 2, 95–103
Article
sac well developed, 15 (14 to 17) mm long, female tail elongate-conoid with pointed terminus; and male with adanal bursa and spicules 21 to 22 mm long (n = 2). The new species comes close in morphology and morphometrics to five known species of the genus, namely N. affinis, N. hexaglyphus, N. persicus, N. taylori, and N. uniformis. Molecular analyses of the partial 18S, D2/D3 expansion segments of the partial 28S and internal transcribed spacer (ITS) revealed this as a new species. The sequences
MEHRAB ESMAEILI,
RAMIN HEYDARI,
WEIMIN YE
Journal of Nematology , ISSUE 3, 268–275
research-article
et al., 2014). The Taizhou and Taiwan populations match well with the original description. However, it is presumed that the population from Hunan is not P. lepidus. The large variation in morphometrics and the photo documentation provided by Yu et al. (2014) are not clear enough to support the identification of this population as P. lepidus. Based on the number of lateral lines, stylet length and the presence/absence of vulval flap, Ghaderi et al. (2014) presented a grouping scheme of
Munawar Maria,
Wentao Miao,
Weimin Ye,
Jingwu Zheng
journal of nematology , 1–13
research-article
J. D. Eisenback,
L. A. Holland,
J. Schroeder,
S. H. Thomas,
J. M. Beacham,
S. F. Hanson,
V. S. Paes-Takahashi,
P. Vieira
journal of nematology , 1–16
research-article
Munawar Maria,
Wentao Miao,
Ruihang Cai,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology , 1–14
research-article
provinces, respectively (Ni and Liu, 2004; Li et al., 2004). Since then, none of the Geocenamus have ever been reported from China; considering the scarce occurrence of the Geocenamus species in China, the detected species was characterized morphologically (using light and scanning microscopy) and molecularly (with 18 S, 28 S, and ITS genes sequences). The morphometrics and morphological characters of the detected species were compared with the related Geocenamus species, and it found that this species
Munawar Maria,
Wentao Miao,
Pablo Castillo,
Jingwu Zheng
Journal of Nematology , 1–13
Research Article
kurosawai sp. n. that are closely related to P. pacificus. Together with five previously described species they form the “pacificus species-complex sensu stricto” that is characterized by all species forming viable, but sterile F1 hybrids indicating reproductive isolation. P. sikae sp. n. and P. kurosawai sp. n. have a gonochorist mode of reproduction and they are described using morphology, morphometrics, mating experiments, and genome-wide sequence analysis. We discuss the extreme diversification in
Kohta Yoshida,
Matthias Herrmann,
Natsumi Kanzaki,
Christian Weiler,
Christian Rödelsperger,
Ralf J. Sommer
Journal of Nematology , ISSUE 3, 355–368
research-article
Hossein Mirbabaei,
Ali Eskandari,
Reza Ghaderi,
Akbar Karegar
Journal of Nematology , 1–14
Article
RAJAN SALALIA,
R. K. WALIA,
VISHAL SINGH SOMVANSHI,
PUNEET KUMAR,
ANIL KUMAR
Journal of Nematology , ISSUE 3, 254–267
Research Article
LIRONG ZHAO,
WEIMIN YE,
MUNAWAR MARIA,
MAJID PEDRAM,
JIANFENG GU
Journal of Nematology , ISSUE 4, 404–417
research-article
Xiphinema barooghii n. sp.
(Figs. 1–3; Tables 1–3).
Table 1
Morphometrics of Xiphinema barooghii n. sp. All measurements are in μm (except L in mm), and in the form: mean ± s.d. (range).
Female
Characters
J1
J2
J3
J4
Holotype
Paratypes
n
3
1
5
5
–
8
L
1.15 ± 0.01 (1.14–1.16)
1.73
2.19 ± 0.12 (2.01–2.31)
3.07 ± 0.17 (2.87–3.34)
4.10
4.02 ± 0.21 (3.67–4.25)
a
44.0 ± 2.3 (41.0–47.0)
41
55.0 ± 3.9 (51.0–62.0)
58.0 ± 5.0 (51.0–64.0)
55
59.0 ± 2.4 (53.0–63.0)
b
3.7 ± 0.1 (3.6
Nasir Vazifeh,
Gholamreza Niknam,
Habibeh Jabbari,
Arezoo Naghavi
Journal of Nematology , 1–17
Research Article
terminus (22.7–29.2 μm, 26.3 μm). The lateral field of J2 had three lines. Cysts (n = 10; Fig. 1C) were lemon-shaped, light to dark brown in color with protruding neck and vulval cone. The cysts had ambifenestrated vulval cone and no bullae was present. Morphometrics included body length excluding neck (370.5–714.4 μm, 555.7 μm); body width (165.6–411.1 μm, 310.9 μm); neck length (36.5–66.3 μm, 49.8 μm); fenestra length (26.3–42.5 μm, 35.8 μm), and fenestra width (19.1–31.5 μm, 23.8 μm). DNA was
Abolfazl Hajihassani,
Bhabesh Dutta,
Ganpati B. Jagdale,
Sergei A. Subbotin
Journal of Nematology , ISSUE 3, 456–458