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original-paper | 27-March-2019

Antibiotic Susceptibility of Cronobacter spp. Isolated from Clinical Samples

but < 2.0 to the genus level if the conditions of species and genus consistency are fulfilled, respectively. Isolates with log-score above 2.3 were used for dendrogram generation if the best three matches indicated the same species. Multilocus sequence typing (MLST). The MLST scheme for the strains investigated was performed previously by Alsonosi et al. (2015) and published in the curated Cronobacter PubMLST open access database (http://www.pubmlst.org/cronobacter). The seven housekeeping genes

ONDŘEJ HOLÝ, ABDLRHMAN ALSONOSI, IGOR HOCHEL, MAGDALÉNA RÖDEROVÁ, SIMONA ZATLOUKALOVÁ, PATRIK MLYNÁRČIK, MILAN KOLÁŘ, JANA PETRŽELOVÁ, AIYDA ALAZRAQ, DITTMAR CHMELAŘ, STEPHEN FORSYTHE

Polish Journal of Microbiology, Volume 68 , ISSUE 1, 5–14

short-communication | 28-April-2020

Extensively Drug-resistant Acinetobacter baumannii Belonging to International Clone II from A Pet Cat with Urinary Tract Infection; The First Report from Pakistan

ZEESHAN TAJ, MUHAMMAD HIDAYAT RASOOL, AHMAD ALMATROUDI, MUHAMMAD SAQALEIN, MOHSIN KHURSHID

Polish Journal of Microbiology, Volume 69 , ISSUE 2, 231–234

original-paper | 08-September-2020

A Polyclonal Spread Emerged: Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from the Intensive Care Unit in a Chinese Tertiary Hospital

). The genes encoding carbapenemases were investigated by polymerase chain reaction (PCR) using a series of primers as previously reported (Queenan and Bush 2007; Nordmann et al. 2011). The amplification products were sent for DNA sequencing (Qingke Biotech, Hangzhou). Isolates genotyping. CRKP isolates in the study were genotyped by multilocus sequence typing (MLST). Seven housekeeping genes including gapA, infB, mdh, pgi, phoE, rpoB, and tonB of K. pneumoniae were amplified and sequenced based on

ZHENGZHENG WANG, FANGYOU YU, XIAOFEI SHEN, MEILAN LI

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 311–319

Research Article

THE APPLICATION OF GENOTYPING AND PHENOTYPING TECHNIQUES FOR EPIDEMIOLOGICAL ANALYSIS OF MICROORGANISMS

. This review discusses and compares methods facilitating bacterial typing at a strain level. Phenotyping methods analysed in this article are: Biotyping, Antimicrobial Susceptibility Typing, Phage Typing and protein-based methods. Genotyping techniques reviewed in this article are based on digestion of genomic DNA, methods using amplification of DNA, and based on sequencing DNA. This would include Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS). Methods used in identification of

Marcin Brzozowski, Paweł Kwiatkowski, Joanna Jursa-Kulesza, Danuta Kosik-Bogacka

Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 3, 353–366

Original Paper | 04-September-2018

Carbapenem-resistant Acinetobacter baumannii from Air and Patients of Intensive Care Units

To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii, the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate A. baumannii isolates were obtained from patients and five isolates of airborne A. baumannii were obtained from the wards’ corridors. Pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were used to analyze the homology relationships of

MEIJIE JIANG, YUNQING MU, NING LI, ZHIJUN ZHANG, SHULIN HAN

Polish Journal of Microbiology, Volume 67 , ISSUE 3, 333–338

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