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Original Paper | 15-March-2016

Recovery of Gram-Positive Cocci and Candida albicans from Peroxygen/Silver-Based Disinfectants

Neutralization method evaluation is an important first step in a disinfectant validation study program. It is also crucial in assessment of the efficiency of microbial recovery media in the presence of a residual biocidal agent. In the present study, four commercially available peroxygen/silver-based disinfectant formulae – intended to be used in a pharmaceutical facility sanitization program – were tested at two dilutions against three Gram-positive cocci and one yeast

Mostafa E. Eissa, Ahmed S. Nouby

Polish Journal of Microbiology, Volume 65 , ISSUE 1, 13–21

Article | 14-October-2020

False reactivity in GTI Pak Plus® ELISA kits due to the presence of anti-mouse antibody in patients' samples

210 patients were evaluated from January 1995 to April 2002; 79 (38%) were found to be positive for HLA- and/or platelet-specific antibodies. Thirty (38%) of these positive samples, as well as ten negative samples that served as controls, underwent HAMA neutralization/inhibition procedures before being retested by ELISA. One (10%) of the control samples was reactive after treatment. When the samples that were positive in routine testing were treated to neutralize/inhibit HAMAs, reactivity was

Miriam Fogg Leach, James P. AuBuchon

Immunohematology, Volume 19 , ISSUE 4, 112–116

Short Communication | 07-June-2016

Enteroviruses Associated with Aseptic Meningitis in Poland, 2011–2014

A 4-year study (2011–2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were posi­tive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively

Magdalena Wieczorek, Agnieszka Figas, Arleta Krzysztoszek

Polish Journal of Microbiology, Volume 65 , ISSUE 2, 231–235

Article | 16-October-2019

Separation of multiple antibodies by adsorption with allogeneic red blood cells

. Routinely, antibody identification practices in a blood bank comprise the testing of a patient’s plasma against reagent RBCs using standard agglutination and indirect antiglobulin methods.1 There are, however, instances when identification of multiple antibodies may be complicated and require additional serologic methods. When the presence of multiple antibodies is suspected, several methods—including neutralization of patient’s plasma, titration, elution, chemical or enzyme treatment of reagent RBCs

E.M. Ekema

Immunohematology, Volume 33 , ISSUE 4, 155–158

Article | 30-November-2019

Inhibition of blood group antibodies by soluble substances

K.M. Byrne, C.M.C. Mercado, T.N. Nnabue, T.D. Paige, W.A. Flegel

Immunohematology, Volume 35 , ISSUE 1, 19–22

Original Paper | 09-March-2018

Isolation of Sabin-like Polioviruses from Sewage in Poland

As a complement to the active search for cases of acute flaccid paralysis, environmental sampling was conducted from January to December 2011, to test for any putative polio revertants and recombinants in sewage. A total of 165 environmental samples were obtained and analyzed for the presence of polioviruses by use of cell culture (L20B, RD and Caco-2) followed by neutralization and reverse-transcription polymerase chain reaction. Out of the 31 CPE positive samples, 26 contained one and 5 two

Agnieszka Figas, Magdalena Wieczorek, Anna Żuk-Wasek, Bogumiła Litwińska

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 89–96

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