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Short Communication

Metagenomic Profiling of the Bacterial Community Changes from Koji to Mash Stage in the Brewing of Soy Sauce

The improvement of soy sauce fermentation is restricted by the insufficient information on bacterial community. In this study, bacterial communities in the koji and mash stage were compared based on next-generation sequencing technology. A total of 29 genera were identi­fied in the koji stage, while 34 in the mash stage. After koji stage, 7 genera disappeared and 12 new genera appeared in the mash stage. The dominant bacteria were Kurthia, Weissella and Staphylococcus in the koji stage and

Hongbin Wang, Quanzeng Wei, Shuqi Gui, Yongrui Feng, Yong Zhang, Yihan Liu, Fuping Lu

Polish Journal of Microbiology , ISSUE 4, 537–541

Short Communication

Changes of Microbial Diversity During Swine Manure Treatment Process

We investigated microbial diversity in a manure storage tank (MST) storing untreated manure and an aeration tank (AT) during swine manure treatment process using the next-generation sequencing in order to find the aeration effect on microbial diversity. Proteobacteria were more abundant in the AT group than in the MST group and may include denitrifying bacteria contributing to nitrous oxide (N2O) emission or aerobic bacteria stimulated by oxygen. The opposite held true for the phyla

Minseok Kim, Jung-Im Yun, Seung-Gun Won, Kyu-Hyun Park

Polish Journal of Microbiology , ISSUE 1, 109–112

Research Article

IMPLEMENTATION OF WHOLE GENOME SEQUENCING FOR BACTERIA GENOTYPING

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology , ISSUE 2, 179–193

research-article

De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

next generation sequencing. The larvae identified as A. pegreffii were used for further analysis. Library construction and next generation sequencing Total RNA (1 μg per each sample set) was prepared by homogenizing approximately 50 larvae per each developmental stage with Trizol, following the manufacturer’s protocol (Invitrogen, USA). Prior to mRNA isolation, total RNA samples were treated with DNAse. And the RNA yield and integrity was measured by NanoDrop 1000 (Thermo Scientific, Wilmington

U-Hwa Nam, Jong-Oh Kim, Jeong-Ho Kim

Journal of Nematology , 1–16

case-report

Pharmacoresistant epilepsy associated with mutations in the KCNB1 and RELN genes. A case report

of seizures, which partially treated AED dosage adjustments. Thus, levetiracetam was discontinued and oxcarbazepine with sodium valproate were added to her treatment plan, without any improvement. At that time, different type of seizures, with tonic-clonic movements, occurred. Due to the combination of speech delay and pharmacoresistant epilepsy, Next Generation Sequencing (NGS) technique with DNA isolation from the patient was performed. Oligonucleotide-based target capture analysis and

Adamantios Katerelos, Nikolaos Zagkos, Dimitra Alexopoulou, Stella Mouskou, Anastasia Korona, Emmanouil Manolakos

Journal of Epileptology , 57–61

research-article

PCR amplification of a long rDNA segment with one primer pair in agriculturally important nematodes

primer pair approach. These ribosomal primer pairs could also be utilized for meta-barcoding by targeting this long rDNA target from environmental nematode DNA samples. The resulting amplicons could be sequenced using different Next Generation Sequencing platforms such as PacBio (Pacific BioSciences, Menlo Park, CA) and Nanopore (Oxford Nanopore Technologies, Oxford, UK) for long reads.

L. K. Carta, S. Li

Journal of Nematology , 1–8

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