Short Communication | 04-December-2017
The improvement of soy sauce fermentation is restricted by the insufficient information on bacterial community. In this study, bacterial communities in the koji and mash stage were compared based on next-generation sequencing technology. A total of 29 genera were identified in the koji stage, while 34 in the mash stage. After koji stage, 7 genera disappeared and 12 new genera appeared in the mash stage. The dominant bacteria were Kurthia, Weissella and Staphylococcus in the koji stage and
Polish Journal of Microbiology, Volume 66 , ISSUE 4, 537–541
Short Communication | 09-March-2018
We investigated microbial diversity in a manure storage tank (MST) storing untreated manure and an aeration tank (AT) during swine manure treatment process using the next-generation sequencing in order to find the aeration effect on microbial diversity. Proteobacteria were more abundant in the AT group than in the MST group and may include denitrifying bacteria contributing to nitrous oxide (N2O) emission or aerobic bacteria stimulated by oxygen. The opposite held true for the phyla
Polish Journal of Microbiology, Volume 67 , ISSUE 1, 109–112
Research Article | 23-May-2019
Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 2, 179–193
original-paper | 31-May-2021
Polish Journal of Microbiology, Volume 70 , ISSUE 2, 273–281
research-article | 16-April-2020
next generation sequencing. The larvae identified as A. pegreffii were used for further analysis.
Library construction and next generation sequencing
Total RNA (1 μg per each sample set) was prepared by homogenizing approximately 50 larvae per each developmental stage with Trizol, following the manufacturer’s protocol (Invitrogen, USA). Prior to mRNA isolation, total RNA samples were treated with DNAse. And the RNA yield and integrity was measured by NanoDrop 1000 (Thermo Scientific, Wilmington
Journal of Nematology, Volume 52 , 1–16
Article | 24-March-2021
Technologia sekwencjonowania następnej generacji (NGS, Next Generation Sequencing), zwana również wysokoprzepustowym sekwencjonowaniem (HTS, High Throughput Sequencing) wraz wielkoskalowymi technikami „omics” stała się powszechnie wykorzystywanym narzędziem w dziedzinie nauk o żywności i żywieniu. W 2009 roku grupa Cifuentesa zaproponowała nową koncepcję oraz praktyczne podejście „foodomiki” do wdrożenia metod „omics” w analizach żywności . Obecnie, foodomika jest definiowana jako
Postępy Mikrobiologii - Advancements of Microbiology, Volume 60 , ISSUE 1, 59–75
case-report | 25-November-2020
of seizures, which partially treated AED dosage adjustments. Thus, levetiracetam was discontinued and oxcarbazepine with sodium valproate were added to her treatment plan, without any improvement. At that time, different type of seizures, with tonic-clonic movements, occurred.
Due to the combination of speech delay and pharmacoresistant epilepsy, Next Generation Sequencing (NGS) technique with DNA isolation from the patient was performed. Oligonucleotide-based target capture analysis and
Journal of Epileptology, Volume 28 , 73–77
original-paper | 17-September-2021
% agarose gel in 1 × TBE buffer.
The 16S rRNA gene amplicon sequencing was performed by the Sentebiolab Biotechnology Company (Turkey) using the Miseq (Illumina) next-generation sequencing platform. The sequences obtained were analyzed using the database on the website (https://www.ezbiocloud.net), and then the sequences were logged in to the GenBank site and accessed “GenBank accession” numbers (Table II). The phylogenetic tree was created by the GGDC web server at http://ggdc.dsmz.de using the
Polish Journal of Microbiology, Volume 70 , ISSUE 3, 297–304
research-article | 03-June-2019
primer pair approach. These ribosomal primer pairs could also be utilized for meta-barcoding by targeting this long rDNA target from environmental nematode DNA samples. The resulting amplicons could be sequenced using different Next Generation Sequencing platforms such as PacBio (Pacific BioSciences, Menlo Park, CA) and Nanopore (Oxford Nanopore Technologies, Oxford, UK) for long reads.
L. K. Carta,
Journal of Nematology, Volume 51 , 1–8