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Research paper | 31-July-2017

Analysis of methionine synthase (rs1805087) gene polymorphism in autism patients in Northern Iran

this study was to analyze the association of MTR A2756G gene polymorphism (rs1805087) and the risk of autism in a population in northern Iran. The prevalence of MTR A2756G polymorphism was determined in 108 children with autism and 130 controls in northern Iran. Genotypes and allele frequencies were determined in patients and controls by polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP). The prevalence of genotype frequencies of AA, AG and GG in autistic children were

Rosa Haghiri, Farhad Mashayekhi, Elham Bidabadi, Zivar Salehi

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 4, 318–323

research-article | 06-November-2020

Morphological and molecular analyses of a Meloidogyne mali population with high intragenomic rRNA polymorphism

conoid and short tapering to a finely rounded or slightly pointed terminus, sometimes more or less rounded. Cuticular constrictions were sometimes present. Hyline tail terminus was variable in length, measuring 6.8 to 12.0 µm. Table 1. Morphometrics of the described M. mali population from Japan compares to a population detected in 2013. M. mali population with high intragenomic rRNA polymorphism M. mali population (Gu et al., 2013) n 20 20 Body length 445  ±  28.3 (401-507) 425

Jianfeng Gu, Yiwu Fang, Lele Liu

Journal of Nematology, Volume 52 , 1–11

research-article | 30-November-2019

Effects of HTR1A rs6295 polymorphism on emotional attentional blink

that the rs6295 polymorphism was not related to vulnerability. In the majority of studies with humans the rs6295 G allele association with threat sensitivity has been shown in clinical or preclinical samples, but the effects are not always clear in studies with the general population (Chipman et al., 2010). However, it is important that data is also collected from neurotypical population subjects to develop early genotyping tests of potential vulnerability. This is obvious from the perspective of

Kadi Tulver, Madis Bachmann, Mariliis Vaht, Jaanus Harro, Talis Bachmann

Acta Neurobiologiae Experimentalis, Volume 80 , ISSUE 4, 389–399

Article | 16-November-2020

ABO genotyping by polymerase chain reaction-restriction fragment length polymorphism

Genotyping enables the identification of both maternally and paternally derived alleles. A number of protocols have been described for the genotyping of the ABO blood group system. Generally, these methods have a number of disadvantages including the use of hazardous reagents, being technically demanding, and the excessive use of materials. In this study, a relatively simple polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method is described. Four different

Nicole A. Mifsud, Albert P. Haddad, Jennifer A. Condon, Rosemary L. Sparrow

Immunohematology, Volume 12 , ISSUE 4, 143–148

Short Communication | 30-March-2017

Use of Amplification Fragment Length Polymorphism to Genotype Pseudomonas stutzeri Strains Following Exposure to Ultraviolet Light A

Changes in ultraviolet light radiation can act as a selective force on the genetic and physiological traits of a microbial community. Two strains of the common soil bacterium Pseudomonas stutzeri, isolated from aquifer cores and from human spinal fluid were exposed to ultraviolet light. Amplification length polymorphism analysis (AFLP) was used to genotype this bacterial species and evaluate the effect of UVA-exposure on genomic DNA extracted from 18 survival colonies of the two strains

Lisa Lombardi, Marina Zoppo, Cosmeri Rizzato, Colin Gerard Egan, Roberto Scarpato, Arianna Tavanti

Polish Journal of Microbiology, Volume 66 , ISSUE 1, 107–111

Report | 16-March-2020

The polymorphism nt 76 in exon 2 of SC is more frequent in Whites than in Blacks

African American donors was tested by polymerase chain reaction (PCR)restriction fragment length polymorphism (RFLP) using the restriction enzyme NlaIII. In selected samples, sequencing of exon 2 was performed. PCR-RFLP results for samples from 100 donors (mostly Caucasian) and 100 African American donors (400 alleles) showed the nucleotide 76T variant had a prevalence of 25 percent in Whites and 5 percent in African Americans. In 11 samples (2 C/C, 3 C/T, and 6 T/T) sequencing of exon 2 confirmed the

Akiko Fuchisawa, Christine Lomas-Francis, Kim Hue-Roye, Marion E. Reid

Immunohematology, Volume 25 , ISSUE 1, 18–19

Article | 20-April-2020

FCGR3B polymorphism in three ethnic Chinese populations

Lixing Yan, Faming Zhu, Lei Jin, Qinfeng Lv, Qihua Fu

Immunohematology, Volume 21 , ISSUE 1, 25–28

Research Article | 17-October-2018

High Mitochondrial Genome Diversity and Intricate Population Structure of Bursaphelenchus xylophilus in Kyushu, Japan

Hanyong Zhang, Erika Okii, Eiji Gotoh, Susumu Shiraishi

Journal of Nematology, Volume 50 , ISSUE 3, 281–302

Article | 14-October-2020

DNA analysis for donor screening of Dombrock blood group antigens

Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes:378 C>T,624 T>C, and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob . We describe here the use of a

Jill R. Storry, Connie M. Westhoff, Dalisay Charles-Pierre, Maria Rios, Kim Hue-Roye, Sunitha Vege, Sandra Nance, Marion E. Reid

Immunohematology, Volume 19 , ISSUE 3, 73–76

Article | 16-November-2020

ABO genotyping - identification of O1, O1*, and O2 alleles using the polymerase chain reaction– sequence specific oligonucleotide (PCR-SSO) technique

ABO polymorphism at the gene level has been investigated by molecular methods, predominantly sequencing and restriction fragment length polymorphism (RFLP). We describe the application of the polymerase chain reaction–sequence specific oligonucleotide (PCRSSO) method, which is considered to be more versatile for large sample numbers, compared with conventional ABO genotyping by PCR-RFLP. PCR-SSO, while maintaining accurate and reliable results, reduces costs and labor. A population of 155

Nicole A. Mifsud, Albert P. Haddad, Jennifer A. Condon, Rosemary L. Sparrow

Immunohematology, Volume 12 , ISSUE 4, 149–153

Research paper | 25-July-2017

BDNF gene polymorphisms and haplotypes in relation to cognitive performance in Polish healthy subjects

Monika Wiłkość, Agnieszka Szałkowska, Maria Skibińska, Ludmiła Zając-Lamparska, Małgorzata Maciukiewicz, Aleksander Araszkiewicz

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 1, 43–52

Article | 14-October-2020

A review of the Knops blood group: separating fact from fallacy

Joann M. Moulds

Immunohematology, Volume 18 , ISSUE 1, 1–8

Review | 29-October-2019

Raph blood group system

Michele Hayes

Immunohematology, Volume 30 , ISSUE 1, 6–10

Original Paper | 26-August-2016

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulo­lytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maxi­mal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between

Chandran Sathesh-Prabu, Young-Keun Lee

Polish Journal of Microbiology, Volume 65 , ISSUE 3, 271–277

Article | 17-November-2020

Loss of the Knops blood group system antigens from stored blood

immunoassay and by flow cytometry measurements. The CR1 expression polymorphism was ascertained by a Hind III restriction fragment length polymorphism analysis. RBC membrane proteins were separated by SDS-PAGE and analyzed for CR1 size by immunoblotting. During the 35-day study interval, no notable decrease was found in RBC-CR1 by flow cytometry. However, CR1 protein was shown to be lost by proteolytic cleavage, as well as by vesiculation. This CR1 protein loss may contribute to the variability of

Joann M. Moulds, L. Lee Brown, Elizabeth Brukheimer

Immunohematology, Volume 11 , ISSUE 2, 46–50

Review | 25-March-2020

The O2 allele: questioning the phenotypic definition of an ABO allele

There are three main alleles in the ABO blood group system, A, B, and O.  The former two alleles encode glycosyltransferases resulting in the wild-type A and B phenotypes, whereas the latter allele does not encode a functional enzyme owing to a frameshift polymorphism in the majority of cases.  Thus the group O phenotype is the absence of A or B sugars.  More than 15 years ago the O2 allele was described; this allele did not feature the usual crippling 261delG polymorphism, which

Mark H. Yazer, Martin L. Olsson

Immunohematology, Volume 24 , ISSUE 4, 138–147

Article | 15-February-2021

An update on the Knops blood group system

expression of E-CR1. Pham et al.8 suggested that the Helgeson phenotype may also be due to the lack of a high-prevalence KN antigen. Patients with anti-KCAM often have the Helgeson phenotype (J.M.M., unpublished data). There is a genetically controlled expression of E-CR1 that can be detected by Southern blot or polymerase chain reaction–restriction fragment-length polymorphism methods. In individuals of European descent, there is good correlation with a HindIII polymorphism (Q981H), but this correlation

J.M. Moulds

Immunohematology, Volume 35 , ISSUE 1, 16–18

Research Article | 22-May-2019

GENETIC DIFFERENTIATION METHODS OF MICROORGANISMS IN THE SOIL – PLANT SYSTEM

Małgorzata Łyszcz, Anna Gałązka

Postępy Mikrobiologii - Advancements of Microbiology, Volume 56 , ISSUE 3, 341–352

Original Paper | 04-December-2017

Study of Patterns and Markers of Human Immune Deficiency Virus -1 (HIV-1) Progression and Unemployment Rate among Patients from Alexandria, Egypt

Middle East and North Africa (MENA) new HIV cases show the highest increase among all regions in the world. Even though Egypt has a low prevalence among the general population (< 0.02%), a national HIV epidemic occurs in certain population risk groups. The current study was conducted to asses clinical and immunological disease progression; following up viral load (VL) and detecting delta-32 CCR5 genotype polymorphism in selected cases, determining unemployment rate and identify predictors of

Faika M. Ghoneim, May M. Raouf, Noha S. Elshaer, Sarah M. Abdelhamid, Reem A. Noor Eldeen

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 519–527

Article | 26-October-2019

Multiplex ligation-dependent probe amplification assay for blood group genotyping, copy number quantification, and analysis of  RH variants

The blood group multiplex ligation-dependent probe amplification (MLPA) is a comprehensive assay, developed for genotyping the majority of clinically relevant blood group antigens in both patients and donors. The MLPA is an easy method to apply and only requires a thermal cycler and capillary electrophoresis equipment. Because the molecular basis of blood group antigens can be a single nucleotide polymorphism, an insertion/deletion polymorphism, or genetic recombination, a single assay such as

Barbera Veldhuisen, C. Ellen van der Schoot, Masja de Haas

Immunohematology, Volume 31 , ISSUE 2, 58–61

Article | 27-December-2020

An update on Rodgers and Chido, the antigenic determinants of human C4

serologic interrelationships. A structural model for antigenic determinants at four polymorphic sites, incorporating sequential and conformational epitopes, was subsequently proposed. Allotype and Rg/Ch data obtained from donors and patients, many with accompanying families, have augmented the model and revealed no exceptions. The antigenic determinants, therefore, make an important contribution to the complex polymorphism of C4.

Carolyn M. Giles

Immunohematology, Volume 5 , ISSUE 1, 1–6

Review | 14-October-2020

The Cromer blood group system: a review

The antigens of the Cromer blood group system reside on decay accelerating factor (DAF), a protein belonging to the regulators of complement activation family. The blood group system consists of eight high-incidence antigens and three low-incidence antigens. The molecular basis for the antigens is known and, with the exception of IFC, each antigen is the product of a single nucleotide polymorphism in the DAF gene and has been localized to one of the four short consensus repeat regions on the

Jill R. Storry, Marion E. Reid

Immunohematology, Volume 18 , ISSUE 4, 95–103

Report | 26-October-2019

High-resolution melting analysis as an alternative method for human neutrophil antigen genotyping

Human neutrophil antigen (HNA)-typed granulocyte panels are widely used to screen for the presence of HNA antibodies and to determine antibody specificity. Many laboratories screen donors for HNA genotypes using low-throughput methods such as allele-specific polymerase chain reaction (PCR), PCR–restriction fragment–length polymorphism, and multiplex PCR. In the present study, we used a high-resolution melting (HRM) analysis to determine HNA genotypes. For the HRM analysis, purified

Kazuta Yasui, Mitsunobu Tanaka, Tomoya Hayashi, Nobuki Matsuyama, Ayumu Kuroishi, Rika. A. Furuta, Yoshihiko Tani, Fumiya Hirayama

Immunohematology, Volume 31 , ISSUE 1, 7–13

Review | 01-December-2019

XG: the forgotten blood group system

Nanette C. Johnson

Immunohematology, Volume 27 , ISSUE 2, 68–71

Review | 01-December-2019

The molecular basis of the LU:7 and LU:–7 phenotypes

reaction, and the products were sequenced. A homozygous novel missense nucleotide change of 1274A>C in exon 10 of LU was observed. This change is predicted to encode Ala at position 425 in place of Glu of the consensus Lu glycoprotein. Based on these results, and an absence of a record of this change in the Single Nucleotide Polymorphism database, Glu425 in the Lu glycoprotein is required for expression of Lu7, and Ala425 is associated with the LU:–7 phenotype. This completes the molecular

Kim Hue-Roye, Marion E. Reid

Immunohematology, Volume 28 , ISSUE 4, 130–131

Report | 11-March-2020

The Indian blood group system

glycoprotein that is encoded by the CD44 gene on chromosome 11 at position p13. The biologic function of CD44 is as a leukocyte homing receptor and cellular adhesion molecule. The Ina and Inb polymorphism represents a 252G>C substitution of CD44, encoding R46P, and lack of IN3 and IN4 results from homozygosity for mutations encoding H85Q and T163R in the CD44 gene. The high-frequency antigen AnWj (901009) has not been assigned to the Indian system, but either is located on an isoform of CD44 or is

Qun Xu

Immunohematology, Volume 27 , ISSUE 3, 89–93

Review | 21-April-2020

Review: Cromer and DAF: role in health and disease

The antigens of the Cromer blood group system are located on the protein decay-accelerating factor (DAF). This system consists of ten high-prevalence and three low-prevalence antigens; the molecular basis for all of these antigens is a single nucleotide polymorphism in the DAF gene. DAF is a 70,000-Da plasma membrane protein that is widely distributed on all blood cells and on endothelial and epithelial tissues. The physiological role of DAF is to inhibit the complement cascade at the level of

Douglas M. Lublin

Immunohematology, Volume 21 , ISSUE 2, 39–47

Review | 26-October-2019

Kidd blood group system: a review

The Kidd blood group system has been recognized as clinically important in red blood cell (RBC) serology since its identification in 1951. Forty years later, the JK glycoprotein was determined to be a product of SCL14A1 and was identical to the urea transport protein UT-B produced by HUT11A. The functional role of the protein as a urea transporter in RBCs and kidney has been well documented. The polymorphism responsible for the antithetical antigens Jka and Jkb was identified in 1994 as c.838G

Janis R. Hamilton

Immunohematology, Volume 31 , ISSUE 1, 29–35

Article | 18-October-2020

Fyx is associated with two missense point mutations in its gene and can be detected by PCR–SSP

Christoph Gassner, Richard L. Kraus, Tadeja Dovc, Susanne Kilga-Nogler, Irene Utz, Thomas Mueller, Friedrich Schunter, Diether Schoenitzer

Immunohematology, Volume 16 , ISSUE 2, 61–67

Report | 01-December-2019

Validation of a blood group genotyping method based on high-resolution melting curve analysis

The detection of polymorphism is the basis of blood group genotyping and phenotype prediction. Genotyping may be useful to determine blood groups when serologic results are unclear. The development and application of different methods for blood group genotyping may be needed as a substitute for blood group typing. The purpose of this study is to establish an approach for blood group genotyping based on a melting curve analysis of realtime polymerase chain reaction (PCR). Using DNA extracted

Tianxiang Gong, Ying Hong, Naihong Wang, Xuemei Fu, Changhua Zhou

Immunohematology, Volume 30 , ISSUE 4, 161–165

Review | 01-December-2019

P1PK: The blood group system that changed its name and expanded

expression but also P1 has been a longstanding enigma. Recently, it was shown that the same A4GALT-encoded galactosyltransferase synthesizes both the P1 and Pk antigens and that a polymorphism in a new exon in this gene predicts the P1 and P2 phenotypes.

Åsa Hellberg, Julia S. Westman, Britt Thuresson, Martin L. Olsson

Immunohematology, Volume 29 , ISSUE 1, 25–33

Research paper | 25-July-2017

Testosterone metabolism: a possible biological underpinning of non-verbal IQ in intellectually gifted girls

range of 10 to18 years and IQ scores higher than 130 were tested. Saliva samples were collected to obtain levels of salivary testosterone. 2D:4D digit ratio was measured on both hands as an indicator of prenatal testosterone. IQ parameters were assessed employing standardized set of tests. The CAG repeat polymorphism in exon 1 of the androgen receptor gene was analyzed to assess the sensitivity of androgen receptor. Testing of between-subjects effects proved significant interactions between right

Jaroslava Durdiaková, Peter Celec, Jolana Laznibatová, Gabriel Minárik, Daniela Ostatníková

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 1, 66–74

Review | 09-October-2019

The Vel blood group system: a review

using different approaches, and all three groups demonstrated that Vel– RBCs lacked SMIM1. This discovery correlated with homozygosity for deletion c.64_60del in SMIM1 and meant that for the first time there was a universal method to screen for Vel– blood donors. This finding was not the whole answer, however, and an explanation behind the variability in antigen strength was later shown to be due to polymorphism in SMIM1 intron 2, a region that is responsible for gene transcription

Jill R. Storry, Thierry Peyrard

Immunohematology, Volume 33 , ISSUE 2, 56–59

original-paper | 05-August-2020

Yeasts Associated with Various Amazonian Native Fruits

CARLOS VEGAS, AMPARO I. ZAVALETA, PAMELA E. CANALES, BRAULIO ESTEVE-ZARZOSO

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 251–261

Article | 09-November-2020

A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population

the HPA-4 antigen system does not involve a common naturally occurring restriction enzyme site. This paper describes a new genotyping method for HPA-4 (polymerase chain reaction–restriction fragment length polymorphism [PCR-RFLP]) that involves restriction enzyme digestion of PCR-amplified genomic DNA using a modified PCR primer to create an artificial TaqI restriction site that is present in the HPA-4a but not in the HPA-4b DNA sequence. The HPA-4 PCR-RFLP method was validated by testing a

Alexander P. Reiner, Gayle Teramura

Immunohematology, Volume 13 , ISSUE 2, 37–43

Case report | 09-October-2019

A LU:-16 individual with antibodies

>T (LU:–16), and a silent polymorphism c.1227G>T. Anti-Lu16 was highly suspected. This would be the fifth case of LU:–16 with antibodies reported, all within women of African heritage with the Lu(a+b−) phenotype. Hemolytic disease of the fetus and newborn was not noted in these cases.

Carole Éthier, Cynthia Parent, Anne-Sophie Lemay, Nadia Baillargeon, Geneviève Laflamme, Josée Lavoie, Josée Perreault, Maryse St-Louis

Immunohematology, Volume 33 , ISSUE 3, 110–113

Report | 17-March-2020

Southeast Asian ovalocytosis is associated with increased expression of Duffy antigen receptor for chemokines (DARC)

-Whitney U test) increase in Fy expression on SAO RBC compared with RBC from individuals without this polymorphism: mean Fy expression (mean fluorescence intensity [MFI]) was 10.12 ± 1.22 for SAO heterozygotes versus an MFI of 8.95 ± 1.1 for individuals without SAO. For reticulocytes the MFI values were 27.61 ± 19.12 for SAO heterozygotes and 16.47 ± 3.81 for controls. SAO is associated with increased and not decreased Fy6 expression so that susceptibility to P. vivax

Ian J. Woolley, Paul Hutchinson, John C. Reeder, James W. Kazura, Alfred Cortés

Immunohematology, Volume 25 , ISSUE 2, 63–66

Research paper | 25-July-2017

Chronotype and sleep quality as a subphenotype in association studies of clock genes in mood disorders

(rs3805148) and the TIM gene (rs2291739), 3) daytime dysfunction with the PER3 gene (rs228727, rs228642, rs10864315), 4) subjective sleep quality with the ARNTL gene (rs11824092, rs1982350), 5) sleep disturbances with the ARNTL gene (rs11600996). We also found the significant epistatic interactions between polymorphism of the PER3 gene (rs2640909) & the CLOCK gene (rs11932595)and following sleep quality variables: sleep duration, habitual sleep efficiency and subjective sleep quality. The present study

Monika Dmitrzak-Węglarz, Joanna Pawlak, Monika Wiłkość, Izabela Miechowicz, Małgorzata Maciukiewicz, Wanda Ciarkowska, Dorota Zaremba, Joanna Hauser

Acta Neurobiologiae Experimentalis, Volume 76 , ISSUE 1, 32–42

Review | 09-October-2019

A Caucasian JK*A/JK*B woman with Jk(a+b–) red blood cells, anti-Jkb, and a novel JK*B allele c.1038delG

antisera. Nevertheless, in RBC genotyping (BioArray HEA BeadChip, Immucor, Warren, NJ) performed in our transfusion service on all patients with alloantibodies, her Kidd typing was JK*A/JK*B based on the Jka/Jkb single nucleotide polymorphism in exon 9 (c.838G>A, p.Asp280Asn). Genomic analysis and cDNA sequencing of her JK*B allele revealed a novel singlenucleotide deletion of c.1038G in exon 11, predicting a frameshift and premature stop (p.Thr346Thrfs*5) after translation of nearly 90 percent of

Glenn Ramsey, Ricardo D. Sumugod, Paul F. Lindholm, Jules G. Zinni, Jessica A. Keller, Trina Horn, Margaret A. Keller

Immunohematology, Volume 32 , ISSUE 3, 91–95

Original Paper | 09-March-2018

Isolation of Sabin-like Polioviruses from Sewage in Poland

different serotypes, yielding a total of 36 PVs. The microneutralization test revealed the presence of 7, 10 and 19 strains belonging to poliovirus serotype 1, 2 and 3, respectively. The genomic variability of 36 poliovirus strains was examined by the restriction fragment length polymorphism assay (RFLP). By combined analyses of two distant, polymorphic segments of the viral genome, one situated in the capsid protein VP1 coding region and the other in the 3D-polymerase coding region, we screened for the

Agnieszka Figas, Magdalena Wieczorek, Anna Żuk-Wasek, Bogumiła Litwińska

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 89–96

Report | 01-December-2019

SC*994C>T causes the Scnull phenotype in Pacific Islanders and successful transfusion of Sc3+ blood to a patient with anti-Sc3  

*994C>T change introduces a restriction enzyme cleavage site for Tsp45I, and polymerase chain reaction (PCR) products from exon 12 were subjected to this PCR–restriction fragment length polymorphism (RFLP) assay. The five samples had the variant SC*994T/T. One sample, from a first cousin of one Marshallese proband, was heterozygous for SC*1514C/T (in the 3′ untranslated region); the other four samples were SC*1514C/C (consensus sequence). Samples from white donors (n = 100) and

Marion E. Reid, Kim Hue-Roye, Randall W. Velliquette, Kathleen Larimore, Sue Moscarelli, Nicolas Ohswaldt, Christine Lomas-Francis

Immunohematology, Volume 29 , ISSUE 2, 69–72

Report | 25-March-2020

Molecular analyses of GYPB in African Brazilians

hemagglutination were selected.  Allele-specific (AS)-PCR and PCR-restriction fragment length polymorphism (RFLP) were used to identify the variant forms of GYPB.  In 13 of 17 S–s– samples (76.5%), both GYPB were deleted.  In 137 of the 148 S–s+ samples (92.6%), the AS-PCR was consistent with the S–s+ phenotype.  In 4 of the S–s– samples (23.5%) and 11 of the S–s+ samples (7.4%), the AS-PCR showed the presence of a GYPB*S allele associated with

Ricardo Omoto, Marion E. Reid, Lilian Castilho

Immunohematology, Volume 24 , ISSUE 4, 148–153

Article | 18-October-2020

Comparison of human platelet antigen (HPA)-1a typing by solid phase red cell adherence to HPA-1 allotypes determined by allelespecific restriction enzyme analysis

forms of the gene using ASRA. Primers (5'- GCTCCAATGTACGGGGTAAACTC-3' and 5'-CAGACCTCCACCTTGTGCTCTATG-3') were designed to amplify the region of DNA that contains the polymorphism and a restriction enzyme (Nci I) was used to cleave the DNA in a predictable manner. Platelet-rich plasma for immunophenotying and anticoagulated whole blood for DNA extraction were obtained from 159 platepheresis donors. Of 159 SPRCA tests, 138 were valid and 21 were invalid due to positive autologous

Michael J. McGann, Jo L. Procter, Junichi Honda, Kazuhiko Matsuo, David F. Stroncek

Immunohematology, Volume 16 , ISSUE 2, 68–73

Report | 26-October-2019

First example of an FY*01 allele associated with weakened expression of Fya on red blood cells

Duffy antigens are important in immunohematology. The reference allele for the Duffy gene (FY) is FY*02, which encodes Fyb. An A>G single nucleotide polymorphism (SNP) at coding nucleotide (c.) 125 in exon 2 defines the FY*01 allele, which encodes the antithetical Fya. A C>T SNP at c.265 in the FY*02 allele is associated with weakening of Fyb expression on red blood cells (RBCs) (called FyX). Until recently, this latter change had not been described on a FY*01 background allele

Patricia A. Arndt, Trina Horn, Jessica A Keller, Rochelle Young, Suzanne M. Heri, Margaret A. Keller

Immunohematology, Volume 31 , ISSUE 3, 103–107

research-article | 09-April-2020

First report of the root-knot nematode, Meloidogyne morocciensis infecting peach in Southern Brazil

, generally continuous, sometimes broken; the phasmids were 29.3 μm apart (25.4-31.9 μm), similar to M. arenaria (Neal, 1889) Chitwood, 1949 and M. incognita (Kofoid and White, 1919; Chitwood, 1949), as observed by Rammah and Hirschmann (1990). The polymorphism analysis revealed the A3N1 phenotype, Est A3 being the phenotype observed for α-esterase with three distinct bands (Rm = 1.11; 1.21; 1.32) (Fig. 1B) and Mdh N1 phenotype corresponding for malate dehydrogenase with only one band (Rm = 1.0) (Fig. 1C

W. R. Silva, C. P. Machaca-Calsin, C. B. Gomes

Journal of Nematology, Volume 52 , 1–3

Article | 16-February-2021

Comparison of ABO genotyping methods: a study of two low-resolution polymerase chain reaction assays in a clinical testing laboratory

peripheral blood were used as a source of genomic DNA (QIAamp DSP; QIAGEN, Valencia, CA). Genomic DNA was polymerase chain reaction (PCR)-amplified and analyzed using two research-use-only genotyping methods: TaqMan-based sequence-specific primer (SSP)-PCR and PCR-restriction fragment length polymorphism (RFLP). SSP-PCR using RBC-FluoGene ABO Basic test kit was performed as per manufacturer’s instructions (inno-train Diagnostik, Kronberg, Germany). The RBC-FluoGene ABO Basic test kit interrogates ABO c

J.A. Keller, T. Horn, S. Scholz, S. Koenig, M.A. Keller

Immunohematology, Volume 35 , ISSUE 4, 149–153

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