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Original Paper | 09-March-2018

Non-invasive Diagnostic of Helicobacter pylori in Stools by Nested-qPCR

The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Rapid Urease Testing (RUT) and histology by Giemsa stain. A fecal sample for nested-qPCR analysis was also obtained. DNA was extracted from the fecal samples, and conventional PCR

María I. Taborda, Gisela Aquea, Yenny Nilo, Karla Salvatierra, Nicolás López, Sergio López, Gustavo Bresky, Juan A. Madariaga, Vittorio Zaffiri, Sergio Häberle, Giuliano Bernal

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 11–18

research-article | 30-November-2018

Identification of Suitable Meloidogyne spp. Housekeeping Genes

emergence of available genomes and transcriptomes are enabling the discovery of plant–nematode interaction mechanisms and nematode loci involved in parasitism (Gleason et al., 2017). Expression of nematode genes is an important factor in determining a role in parasitism. However, housekeeping (HK) genes for RKN have not been defined and validated, reducing the accuracy and credibility of gene expression studies. Reverse transcription quantitative PCR (RT-qPCR) is a standard for quantifying mRNA because

Weiming Hu, Peter M. DiGennaro

journal of nematology, Volume 51 , 1–11

Original Paper | 28-June-2017

Molecular Study of Indigenous Bacterial Community Composition on Exposure to Soil Arsenic Concentration Gradient

Community structure of bacteria present in arsenic contaminated agricultural soil was studied with qPCR (quantitative PCR) and DGGE (Denaturing Gradient Gel Electrophoresis) as an indicator of extreme stresses. Copy number of six common bacterial taxa (Acidobacteria, Actinobacteria, α-, β- and γ-Proteobacteria, Firmicutes) was calculated using group specific primers of 16S rDNA. It revealed that soilcontaminated with low concentration of arsenic was dominated by both

Semanti Basu, Tanima Paul, Priya Yadav, Abhijit Debnath, Keka Sarkar

Polish Journal of Microbiology, Volume 66 , ISSUE 2, 209–221

Original Paper | 04-December-2017

Identification of Lactobacillus delbrueckii and Streptococcus thermophilus Strains Present in Artisanal Raw Cow Milk Cheese Using Real-time PCR and Classic Plate Count Methods

way and that genomic DNA solutions were free of PCR inhibitors. These methods revealed the presence of L. delbrueckii and S. thermophilus. The real-time PCR enabled quantification with a detection of 101–103 CFU/g of product. qPCR-standard curves were linear over seven log units down to 101 copies per reaction; efficiencies ranged from 77.9% to 93.6%. Cheese samples were analysed with plate count method and qPCR in parallel. Compared with the classic plate count method, the newly developed

Milena A. Stachelska

Polish Journal of Microbiology, Volume 66 , ISSUE 4, 491–499

research-article | 30-November-2018

Temporal expression patterns of Pasteuria spp. sporulation genes

mortar and pestle with 2-ml of TRIzol (Invitrogen, Waltham, MA). RNA extraction was performed as per the manufacturer’s instructions. Purified RNA from infested plant roots was synthesized into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). Each independent replicate for the seven times points evaluated was replicated in three 10 µl qPCR reactions using iTaqTM Universal SYBR® green Supermix (Bio-Rad, Hercules, CA). The qPCR experiments were performed using the ABI 7500 Real-Time

Ruhiyyih Dyrdahl-Young, Weiming Hu, Peter DiGennaro

Journal of Nematology, Volume 51 , 1–8

research-article | 30-November-2020

An overview of technologies and devices against COVID-19 pandemic diffusion: virus detection and monitoring solutions

tests are convenient and rapid, the technological issues limit their applicability. Since these tests require to check the antibodies produced by the human organism against SARS-CoV-2 following symptom onset, they take a substantial amount of time. Moreover, SARS-CoV-2 antibodies have significant cross-reactivity with the antibodies generated by other coronaviruses. For this reason, nucleic acid-based real-time reverse transcription Polymerase Chain Reaction (PCR) (RT-qPCR) assays are worldwide

R. de Fazio, A. Sponziello, D. Cafagna, R. Velazquez, P. Visconti

International Journal on Smart Sensing and Intelligent Systems, Volume 14 , ISSUE 1, 1–28

research-article | 30-November-2019

Lovastatin alters neurotrophin expression in rat hippocampus-derived neural stem cells in vitro

cells were considered positive. Real-time RT-qPCR RT-qPCR was carried out using extracted cDNA from control and treatment groups. Total RNA of hippocampal tissues was isolated by TRIzol® (Invitrogen/Life Technologies). We used 1,000 ng of purified RNA to synthesize 20 μl of cDNA according to a Revert Aid™ First Strand cDNA Synthesis Kit (Fermentas, Germany). The cDNA was then used to quantify mRNA levels of the neurotrophins Bdnf, Gdnf, Cntf, Ngf, NT-3 and NT-4. Glyceraldehyde 3-phosphate

Farzaneh Fakheri, Alireza Abdanipour, Kazem Parivar, Iraj Jafari Anarkooli, Hossein Rastegar

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 4, 413–420

original-paper | 28-June-2019

In situ Impact of the Antagonistic Fungal Strain, Trichoderma gamsii T30 on the Plant Pathogenic Fungus, Rhizoctonia solani in Soil

evaluate the in situ impact of the biocontrol strain on the population density of the plant pathogenic R. solani AG 2-2 strain G6 in the soil. For this purpose, microcosms containing the autoclaved or non-autoclaved soils were used. The microcosms were inoculated with both the pathogen (R. solani AG 2-2 strain G6) and the antagonist (T. gamsii strain T30) in different ratios. The population densities of the pathogenic fungus were followed up to day 25 using real-time polymerase chain reaction (qPCR) by

MUHAMMAD ANEES, MUHAMMAD ABID, SOBIA CHOHAN, MUHAMMAD JAMIL, NADEEM AHMED, LIXIN ZHANG, EUI SHIK RHA

Polish Journal of Microbiology, Volume 68 , ISSUE 2, 211–216

Original Paper | 30-June-2018

The Heavy-Metal Resistance Determinant of Newly Isolated Bacterium from a Nickel-Contaminated Soil in Southwest Slovakia

MATEJ REMENÁR, ANNA KAMLÁROVÁ, JANA HARICHOVÁ, MARCEL ZÁMOCKÝ, PETER FERIANC

Polish Journal of Microbiology, Volume 67 , ISSUE 2, 191–201

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