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original-paper | 08-September-2020

Characterization of Two Macrolide Resistance-Related Genes in Multidrug-Resistant Pseudomonas aeruginosa Isolates

annealing temperature (Table I), and 1 min 45 s at an extension temperature of 72°C; and a final extension step for 10 min at 72°C. Positive amplification products were verified by sequencing with an ABI 3730 automated sequencer (Shanghai Sunny Biotechnology Co., Ltd., Shanghai, China), and the sequencing results were compared with the reference resistance gene sequences using BLAST algorithms. The amplicons of the two target genes (mphE and msrE) with its promoter regions were digested with the

QING CHEN, WEI LU, DANYING ZHOU, GUOTONG ZHENG, HONGMAO LIU, CHANGRUI QIAN, WANGXIAO ZHOU, JUNWAN LU, LIYAN NI, QIYU BAO, AIFANG LI, TENG XU, HAILI XU

Polish Journal of Microbiology, Volume 69 , ISSUE 3, 349–356

research-article | 30-November-2020

Biology, pathotype, and virulence of Globodera rostochiensis populations from Kenya

same variety was similar. Both G. rostochiensis ‘HAR1’ and ‘Ecosse’ had similar development pattern in the roots of the resistant potato ‘Laura’. On the resistant cultivar, no females, but males were detected in the roots and the soils. Failure of females to develop in the roots of ‘Laura’ can be attributed to the presence of the H1 resistance gene that confers resistance to pathotypes Ro1 and Ro4 (Bakker et al., 2006; Dalamu et al., 2012). The resistance response involves a hypersensitive reaction

James M. Mwangi, Grace N. Mwangi, Maria R. Finckh, Sebastian Kiewnick

Journal of Nematology, Volume 53 , 1–13

Research Article | 31-May-2018

Influence of Temperature on Susceptibility of CVS. Tifguard and Georgia-06G Peanut to Meloidogyne arenaria

were observed. The optimum temperature for nematode infection and development was 31°C in Georgia-06G. In summary, it is unlikely that high soil temperatures would lessen the effectiveness of the nematode resistance gene in Tifguard.

Weimin Yuan, C. C. Holbrook, Y. Chu, P. Ozias-Akins, D. W. Dickson

Journal of Nematology, Volume 50 , ISSUE 1, 33–40

Article | 21-July-2017

Reproduction of Meloidogyne incognita Race 3 on Flue-cured Tobacco Homozygous for Rk1 and/or Rk2 Resistance Genes

Most commercial tobacco cultivars possess the Rk1 resistance gene to races 1 and 3 of Meloidogyne incognita and race 1 of Meloidogyne arenaria, which has caused a shift in population prevalence in Virginia tobacco fields toward other species and races. A number of cultivars now also possess the Rk2 gene for root-knot resistance. Experiments were conducted in 2013 to 2014 to examine whether possessing both Rk1 and Rk2 increases resistance to a variant of M. incognita race 3 compared to

JILL R. POLLOK, CHARLES S. JOHNSON, J. D. EISENBACK, T. DAVID REED

Journal of Nematology, Volume 48 , ISSUE 2, 79–86

Original Paper | 30-June-2018

The Heavy-Metal Resistance Determinant of Newly Isolated Bacterium from a Nickel-Contaminated Soil in Southwest Slovakia

[CP001068] with 99 and 100% similarities, respectively. In addition, the whole nccA-like heavy-metal resistance gene sequence (3,192 bp), marked as MR-CH-I2-nccA [KR476581], was obtained and on the base of phylogenetic analysis its assignment was confirmed to MULTISPECIES: cation efflux system protein CzcA [WP_004635342] from Burkholderiaceae with 98% similarity. Furthermore, although the bacterium carried one high molecular plasmid of about 50 kb in size, nccA-like gene was not located on this plasmid

MATEJ REMENÁR, ANNA KAMLÁROVÁ, JANA HARICHOVÁ, MARCEL ZÁMOCKÝ, PETER FERIANC

Polish Journal of Microbiology, Volume 67 , ISSUE 2, 191–201

Research Article | 03-December-2018

Broad-based root-knot nematode resistance identified in cowpea gene-pool two

Cowpea (Vigna unguiculata L. Walp) is an affordable source of protein and strategic legume crop for food security in Africa and other developing regions; however, damage from infection by root-knot nematodes (RKN) suppresses cowpea yield. The deployment through breeding of resistance gene Rk in cowpea cultivars has provided protection to cowpea growers worldwide for many years. However, occurrence of more aggressive nematode isolates threatens the effectiveness of this monogenic resistance. A

Arsenio D. Ndeve, William C. Matthews, Jansen R. P. Santos, Bao Lam Huynh, Philip A. Roberts

Journal of Nematology, Volume 50 , ISSUE 4, 545–558

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