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Original Paper | 09-March-2018

High-Throughput Sequencing Analysis of Endophytic Bacteria Diversity in Fruits of White and Red Pitayas from Three Different Origins

second-generation high-throughput sequencing technology. Large number of endophytic bacteria were detected and 22 phyla, 56 classes, 81 orders, 122 families and 159 genera were identified. Endophytic bacteria diversity was uneven among pitaya fruits from different origins and bacteria structure was different between white pitaya group and red pitaya group. Phylum Bacteroidetes, classes Bacteroidia and Coriobacteriia, orders Bacteroidales and Coriobacteriales, families Prevotellaceae, Bacteroidaceae

Zhen Ren, Shukun Tang, Yi Jiang, Mingxing Jiang, Shangyong Zheng, Wenjing Liu, Zhili Yang, Shuping Sang, Zebin Chen, Tiyuan Xia, Min Yin

Polish Journal of Microbiology, Volume 67 , ISSUE 1, 27–35

Article | 01-March-2016


For the wireless sensor networks (WSNs), MAC layer protocol, relating to system throughput, involving functions of system, concerning the consumption of system energy efficiency, etc. other system indicators, is very important. With the development of WSN, involving vary businesses, the amount and style of data which the sensor nodes detecting is large and divertive. Based on three-dimensional probability CSMA protocol, the proposed protocol, multi-channel three-dimensional probability CSMA

Yifan Zhao

International Journal on Smart Sensing and Intelligent Systems, Volume 9 , ISSUE 1, 334–352

short-communication | 28-June-2019

Bacterial Diversity in Soybean Rhizosphere Soil at Seedling and Mature Stages

nodules of leguminous plants lacking hydrogenase. Experiments have shown that bacteria remove hydrogen (Mclearn and Dong 2002), and these bacteria were beneficial to the growth of plants (Dong et al. 2003; Abdellatif et al 2017). Therefore, studying the rhizosphere microbial diversity of legumes will improve our understanding of microbes that promote the growth of legumes. Accordingly, in this study, Illumina high-throughput sequencing was used to investigate the microbial communities of rhizosphere


Polish Journal of Microbiology, Volume 68 , ISSUE 2, 281–284

Research Article | 01-March-2017


In wireless Ad Hoc networks, large number and flexible mobility of terminals lead to the rarity of wireless channel resources. Also the hidden and exposed terminal problem exists in the Ad Hoc network which is the major factors restricting its development and applying. Considering these factors, this paper proposes a new CSMA protocol: multi-channel two-dimensional probability CSMA for wireless Ad Hoc network protocol based on three-way handshake mechanism, and analyzes the system throughput

Yifan Zhao, Hongwei Ding, Yingying Guo, Jing Nan, Shengjie Zhou, Shaowen Yao, Qianlin Liu

International Journal on Smart Sensing and Intelligent Systems, Volume 10 , ISSUE 1, 88–107

original-paper | 31-May-2021

Diversity of Endophytic Fungal Community in Leaves of Artemisia argyi Based on High-throughput Amplicon Sequencing

in A. argyi based on high-throughput sequencing have not yet been reported. Therefore, to evaluate the influence of endophytic fungi on the growth and quality of A. argyi, the present study employed high-throughput amplicon sequencing technology to compare and analyze the diversity and composition of endophytic fungal community structure in the leaves of A. argyi cultivated in different regions in China. Besides, the degree of health and biocontrol application potential of A. argyi was also


Polish Journal of Microbiology, Volume 70 , ISSUE 2, 273–281

research-article | 30-November-2018

Developing a real-time PCR diagnostic method for a potential threat to chrysanthemum, Paratylenchus dianthus

. kumamotoensis are known to inhabit in many chrysanthemum growing areas (Iwahori et al., 2008) and Pratylenchus may cause chronic damage to chrysanthemum (Kobayashi, 1995). Chemical treatments including nematicides, such as fosthiazate, are popular means to control nematode damage in Okinawa, however, nematode diagnosis in a chrysanthemum field is not quick and easy. Koyama et al. (2016) reported a low cost and high-throughput approach to quantify Pratylenchus spp. with the real-time PCR method by using pre

Masanori Kawanobe, Koki Toyota, Hidehito Uchihara, Mikoto Takae

Journal of Nematology, Volume 51 , 1–11

Article | 30-November-2018

Research on Trust-Role Access Control Model in Cloud Computing

experiments in the local business system. The cloud platform is built through Hadoop architecture. Firstly, the local database is migrated to the distributed data base HBase, and then the access control model is applied in the local business system. In other words, the user’s identity, credibility and other information are verified at the same time. Finally, the advantages of this model in system throughput and data storage cost are obtained through simulation experiments. A. Construction of hadoop cloud

Yuchen Wu, Pingping Liu

International Journal of Advanced Network, Monitoring and Controls, Volume 4 , ISSUE 2, 75–80

Research Article | 15-February-2020

Analysis of Frequency Channel Division Strategy for CSMA/CA with RTS/CTS Mechanism

In this work we study the collision probability, saturation throughput and statistical delay for the carrier sense multiple access collision avoidance (CSMA/CA) protocol with request to send and clear to send (RTS/CTS) mechanism in the case of frequency channel division. We propose in this paper a modified version of CSMA/CA-RTS/CTS to be compatible with thechannelrepartitiontechniqueandweprovethatanimportant gain is introduced in terms of system performance especially for loaded networks

Baher MAWLAWI, Jean-Baptiste DORÉ, Nikolai LEBEDEV, Jean-Marie GORCE

International Journal on Smart Sensing and Intelligent Systems, Volume 7 , ISSUE 5, 1–5

Research Article | 21-April-2017

Research of influence of time of reaction of the driver on the calculation of the capacity of the highway

In the work we performed a review of studies of foreign scholars on changing the reaction time of the driver depending on various road conditions, namely the change in the response time when using the traffic light regulation. Earlier by the authors of this article have already been carried out research in the field of throughput of a site of a highway with traffic regulation, which showed that this value depends on the time of reaction of the driver. In this article the estimation of how much


Transport Problems, Volume 10 , ISSUE 3, 53–59

Article | 01-June-2016


Yifan Zhao, Shengjie Zhou, Hongwei Ding, Zhijun Yang, Qianlin Liu

International Journal on Smart Sensing and Intelligent Systems, Volume 9 , ISSUE 2, 845–863

Article | 17-February-2021

A resource-conserving serologic and high-throughput molecular approach to screen for blood donors with an IN:−5 phenotype

S.R. Joshi, S.B. Senjaliya, K. Srivastava, W.A. Flegel

Immunohematology, Volume 36 , ISSUE 4, 129–132

Article | 01-September-2015


drop, throughput, energy consumption, residual energy and network lifetime. The proposed scheme achieves better performance than the existing EEHA and SMART schemes.

M. Roseline Juliana, S. Srinivasan

International Journal on Smart Sensing and Intelligent Systems, Volume 8 , ISSUE 3, 1748–1767

Article | 01-December-2012


dynamically and efficiently, leading to improving the channel efficiency of the networks. Simulation results show that compared with the existing protocols, the proposed protocol can achieve a high throughput, a low packet drop ratio, and a low transmission delay.

Guangyu Fan, Jian Li, Wenhong Liu, Yang Zhao

International Journal on Smart Sensing and Intelligent Systems, Volume 5 , ISSUE 4, 879–895

Report | 25-March-2020

Principles of PCR-based assays

molecular testing, popular because they analyze multiple nucleotides in a single assay and have a high-throughput potential.  This review briefly describes the principles of PCR-based assays that are commonly used in transfusion medicine.

Kim Hue-Roye, Sunitha Vege

Immunohematology, Volume 24 , ISSUE 4, 170–175

original-paper | 28-June-2019

Gastric Microbiota Alteration in Klebsiella pneumoniae-Caused Liver Abscesses Mice

Specific Pathogen Free (SPF) animal house. All the animal experiments in this study were conducted in accordance with the National Research Council Guide for Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of the First Affiliated Hospital of Zhejiang Medical University (2013–012) and the Animal Welfare and Ethical Committee of Hebei University (2018026). DNA extraction and high-throughput sequencing analysis. After mice sacrificed, the stomachs of


Polish Journal of Microbiology, Volume 68 , ISSUE 2, 247–254

Article | 26-October-2019

Mass-scale donor red cell genotyping using real-time array technology

Gregory A. Denomme, Michael J. Schanen

Immunohematology, Volume 31 , ISSUE 2, 69–74

research-article | 18-March-2020

Transcriptomic analysis of Bursaphelenchus xylophilus treated by a potential phytonematicide, punicalagin

Qun-Qun Guo, Gui-Cai Du, Ting-Ting Zhang, Mei-Juan Wang, Chao Wang, Hong-Tao Qi, Rong-Gui Li

Journal of Nematology, Volume 52 , 1–14

Article | 26-October-2019

An overview of the use of SNaPshot for predicting blood group antigens

based on the population or laboratory reality and the purpose of the genotyping. Unlike high-throughput genotyping strategies that are provided as commercial platforms, the SNPs can be chosen to best meet the needs of the user, and the interpretation of the results do not depend on the manufacturer.

Flavia R.M. Latini, Lilian M. Castilho

Immunohematology, Volume 31 , ISSUE 2, 53–57

Research paper | 01-June-2011

A Novel Biased Energy Distribution (BED) Technique for Cluster-Based Routing in Wireless Sensor Networks

solution prioritizing the network into higher and lower energy nodes. The aim of this approach is to ensure well balanced energy consumption in order to maximize network lifetime. It is shown by simulation that the proposed technique exhibits better performance when compared to existing clustering routing techniques in terms of throughput, network lifetime and energy consumption.

A. F. Salami, H. Bello-Salau, F. Anwar, A. M. Aibinu

International Journal on Smart Sensing and Intelligent Systems, Volume 4 , ISSUE 2, 161–173

Research Article | 23-May-2019


molecular microbiology, thus providing modern tools for the identification of sources and routes of infection. Whole genome sequencing and other high-throughput typing methods are becoming increasingly popular and, thanks to their high resolution, they are ideal tools for comparative analysis of bacteria. This study reviews the methods most commonly used in the molecular typing of bacteria, including those which are in the development stage and may be the main tool in microbial typing in the future.

Daria Artyszuk, Tomasz Wołkowicz

Postępy Mikrobiologii - Advancements of Microbiology, Volume 57 , ISSUE 2, 179–193

Review | 20-March-2020

Detection and identification of platelet antibodies and antigens in the clinical laboratory

antibody tests. Fueled by development of PCR and determination of the molecular basis of the PlA1 human platelet antigen (HPA), serologic platelet typing has now been replaced by genotyping of DNA. Allele-specific PCR, melting curve analysis, and 5′-nuclease assays are now evolving into more high-throughput molecular tests. Laboratory testing for the diagnosis of immune platelet disorders has advanced considerably from its humble beginnings.

Brian R. Curtis, Janice G. McFarland

Immunohematology, Volume 25 , ISSUE 3, 125–135

Article | 05-September-2013


accordance with the real time traffic detected, including traffic volume, waiting time and vehicle density. Subsequently, the optimal traffic light length can be determined according to the local traffic and predicted traffic data. Simulation results demonstrate that the proposed scheme can achieve much higher performance, in terms of throughput and average waiting time. We also implement proposed scheme into our WSN-based ITS project, iSensNet, and the result shows that our scheme is effective and

Binbin Zhou, Jiannong Cao, Jingjing Li

International Journal on Smart Sensing and Intelligent Systems, Volume 6 , ISSUE 4, 1559–1581

Research Article | 01-September-2017


. In the proposed system, the above methodology has been extended to implement a memory efficient FPGA-hardware based Network Intrusion Detection System (NIDS) to speed up network processing. The pattern matching in Intrusion Detection Systems (IDS) is done using SNORT to find the pattern of intrusions. A Finite State Machine (FSM) based Processing Elements (PE) unit to achieve minimum number of states for pattern matching and bit wise early intrusion detection to increase the throughput by

M.Anto Bennet, S. Sankaranarayanan, M. Deepika, N. Nanthini, S. Bhuvaneshwari, M. Priyanka

International Journal on Smart Sensing and Intelligent Systems, Volume 10 , ISSUE 5, 101–122

Original Paper | 10-December-2018

Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in


Polish Journal of Microbiology, Volume 67 , ISSUE 4, 417–430

Report | 26-October-2019

High-resolution melting analysis as an alternative method for human neutrophil antigen genotyping

Human neutrophil antigen (HNA)-typed granulocyte panels are widely used to screen for the presence of HNA antibodies and to determine antibody specificity. Many laboratories screen donors for HNA genotypes using low-throughput methods such as allele-specific polymerase chain reaction (PCR), PCR–restriction fragment–length polymorphism, and multiplex PCR. In the present study, we used a high-resolution melting (HRM) analysis to determine HNA genotypes. For the HRM analysis, purified

Kazuta Yasui, Mitsunobu Tanaka, Tomoya Hayashi, Nobuki Matsuyama, Ayumu Kuroishi, Rika. A. Furuta, Yoshihiko Tani, Fumiya Hirayama

Immunohematology, Volume 31 , ISSUE 1, 7–13

research-article | 30-November-2019

Machine-to-machine and cell-to-cell traffic handling using relay and carrier aggregation prioritize on LTE-A PRO network

PR-M2M average throughput. Channel model The channel gain on the communication link between any two nodes denoted by i and j using a subcarrier k is (1) G i , j , k dB = − ( λ log d i , j + κ + β i , j , k ) + 10 log R i , j , k , (1)where λ is the path loss exponent, d i,j denotes distance in km between nodes i and j, κ is propagation loss, and β i,j,k represents log-normal shadowing with a standard deviation of 8 dB and zero mean. The Rayleigh

Vuyo Pana, Vipin Balyan, Ben Groenewald

International Journal on Smart Sensing and Intelligent Systems, Volume 13 , ISSUE 1, 1–9

Report | 25-March-2020

Value of DNA-based  assays for donor screening and  regulatory issues

antibody is not always commercially available, and that it may be limited in volume, weakly reactive, or costly.  These scenarios can make it difficult to screen for large numbers of antigen-negative blood donors.  The knowledge of the molecular bases of blood group antigens makes it possible to screen donors to predict their antigen status.  High-throughput platforms provide a means to test relatively large numbers of donors, thereby opening the door to change the way antigen-negative

Donna Strauss, Marion E. Reid

Immunohematology, Volume 24 , ISSUE 4, 175–179

Article | 21-July-2017

Assessment of Globodera pallida RNA Extracted from Solanum Roots

The introduction of high-throughput sequencing technologies has made transcriptome analyses of plant–pathogen interactions almost routine. Nevertheless, it is still challenging to obtain RNA from populations made up of two species. An RNA extraction method that worked well on free-living Caenorhabditis elegans failed when applied to isolated Globodera pallida J2 larva. Furthermore, alternative protocols that extracted RNA from free-living J2 larva produced less satisfactory


Journal of Nematology, Volume 49 , ISSUE 1, 12–20

original-paper | 08-September-2020

Characteristics and Diversity of Endophytic Bacteria in Endangered Chinese Herb Glehnia littoralis Based on Illumina Sequencing

communities in all the samples are listed in Table II. Among the samples, the bacterial communities’ richness and diversity in roots were highest, followed by stem and leaf. In addition, the ACE and Chao1 richness values and Shannon index of the root were significantly higher than that of stem and leaf. Bacterial taxonomic analysis at phylum level. High-throughput sequences annotated to the Bacteria domain were identified into 29 bacterial phyla. The relative community abundance on the phylum level of the


Polish Journal of Microbiology, Volume 69 , ISSUE 3, 283–291

original-paper | 30-November-2018

Microbiota and Chemical Compounds in Fermented Pinelliae Rhizoma (Banxiaqu) from Different Areas in the Sichuan Province, China

reasons: firstly, our samples were dry fermented Pinelliae Rhizoma, and some bacteria that grow at higher humidity or occurred occasionally in the fermentation process of Pinellia ternate could not be reliably detected, especially those which do not produce spores. Secondly, high-throughput sequencing can supply more comprehensive information about the microbiota compared to the culture method. Finally, the results of our study were summarized from three fermented Pinellia ternate, that ensured most


Polish Journal of Microbiology, Volume 68 , ISSUE 1, 83–92

research-article | 30-November-2019

Implementation of a low cost, solar charged RF modem for underwater wireless sensor networks

powered by the solar cells during the day and through the charged batteries during night, which means it can be used to create a sustainable underwater monitoring network. Results show that the total communication range almost doubles for each added node when the handover mode is enabled. Additionally, experiments were done in order to test the performance of the overall network in terms of overall throughput. The rest of the paper is organized as follows. The second section presents the modem design

Mohammad M. Abdellatif, Salma M. Maher, Ghazal M. Al-sayyad, Sameh O. Abdellatif

International Journal on Smart Sensing and Intelligent Systems, Volume 13 , ISSUE 1, 1–11

research-article | 30-November-2020

TDMA-clustering-based approach to avoid the reader-to-tag collision problem during the stocktaking process

checks its queues and decides accordingly. If the first queue is empty, the reader chooses a different channel to operate on and broadcasts it to its neighbors beforehand, otherwise, it waits for an END signal from its neighbors to operate at a different time. Despite the improvement of both the throughput and efficiency of the RFID system, this solution relies on an overhead created by the exchanged messages, which can affect the delay. Furthermore, the authors did not address collisions among

Sara El Ouadaa, Slimane Bah, Abdelaziz Berrado

International Journal on Smart Sensing and Intelligent Systems, Volume 14 , 1–14

research-article | 30-November-2018

Cell-based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells

medium, repeated observation is not conducive to cell growth. In addition, during the immunofluorescence staining, the microplate should be rinsed as gently as possible to avoid cell detachment from the microplate. With the development of combinatorial chemistry and diverse compound libraries, the pharmaceutical industry and academia have used high-throughput screening (HTS) technology to screen thousands of novel compounds. HTS analysis can be divided into two categories, biochemical assays, which

Kun Zhang, Bin Li, Peifang Li, Xiaoli Yang, Huixian Cui, Xiaoyun Liu

Acta Neurobiologiae Experimentalis, Volume 79 , ISSUE 3, 303–309

research-article | 30-November-2020

Reclaimed desert habitats favor entomopathogenic nematode and microarthropod abundance compared to ancient farmlands in the Nile Basin

families detected in the two ecoregions. Results The high-throughput sequencing produced two datasets, one based on ITS2 targeting nematodes and the other on the D3-D5 region of 28 S rDNA targeting microarthropods. The ITS2 revealed 8,208,384 reads of which 43% (3,522,968) passed the quality filters and were denoised, merged and characterized as non-chimeric. 44,483 unique amplicon sequence variants (ASVs) were recovered from forty-nine phyla, with 2.1% (946 ASVs) assigned to phylum Nematoda. By

Alexandros Dritsoulas, Fahiem E. El-Borai, Ibrahim E. Shehata, Mostafa M. Hammam, Ramadan M. El-Ashry, Moawad M. Mohamed, Mahfouz M. Abd-Elgawad, Larry W. Duncan

Journal of Nematology, Volume 53 , 1–13

Article | 26-October-2019

Blood group genotyping: the power and limitations of the Hemo ID Panel and MassARRAY platform

a set of genotyping assays that predict the phenotype for 101 antigens from 16 blood group systems. These assays involve three fundamental stages: multiplex target-specific polymerase chain reaction amplification, allele-specific single base primer extension, and MALDI-TOFMS analysis using the MassARRAY system. MALDI-TOF MS–based genotyping has many advantages over alternative methods including high throughput, high multiplex capability, flexibility and adaptability, and the high level of

Rhiannon S. McBean, Catherine A. Hyland, Robert L. Flower

Immunohematology, Volume 31 , ISSUE 2, 75–80

research-article | 30-November-2020

Energy harvested end nodes and performance improvement of LoRa networks

PHY layer parameters to extend the network’s coverage and throughput simultaneously. Energy harvesting and scheduling The requirements of high-speed networking in all the sectors is putting a burden in the form of resources to store and means to conserve energy, which is further growing due to massive sizes (Khargharia et al., 2007). The LoRa flexibility is limited when the devices are powered by energy sources (batteries). The deployment of such devices further limits the performance of the

Gunjan Gupta, Robert Van Zyl

International Journal on Smart Sensing and Intelligent Systems, Volume 14 , ISSUE 1, 1–15

Article | 17-February-2021

Concordance of two polymerase chain reaction–based blood group genotyping platforms for patients with sickle cell disease

alloanti-C. Meyer et al.19 also found high concordance between serotyping and a high-throughput matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry assay for Kell, Kidd, and Duffy antigens (the lowest concordance reported was 99.2%). They detected new alleles using their molecular platform. Our study showed that identified discrepancies between the two assays primarily occurred in the highly variable Rh blood group system. In some cases, the two assays interrogated different

C.A. Sheppard, N.L. Bolen, G. Meny, M. Kalvelage, G. Ochoa-Garay

Immunohematology, Volume 36 , ISSUE 4, 123–128

Report | 09-October-2019

Red blood cell phenotype prevalence in blood donors who self-identify as Hispanic

Molecular genotyping platforms provide a quick, high-throughput method for identifying red blood cell units for patients on extended phenotype-matching protocols, such as those with sickle cell disease or thalassemia. Most of the antigen prevalence data reported are for non-Hispanic populations. Therefore, this study sought to determine the phenotype prevalence in a single blood center’s Hispanic population and to compare those results with previously reported rates in non-Hispanic donor

Chelsea A. Sheppard, Nicole L. Bolen, Beth Eades, Gorka Ochoa-Garay, Mark H. Yazer

Immunohematology, Volume 33 , ISSUE 3, 119–124

Report | 20-March-2020

A simple screening assay for the most common JK*0 alleles revealed compound heterozygosity in Jk(a–b–) probands from Guam

screening assay was cost-efficient when compared with DNA sequencing costs. Furthermore, selection of the more common JK*0 mutations was a practical approach that resulted in rapid identification of the genetic bases behind the Jk(a–b–) phenotypes in this unusual family. Although an obvious target for eventual inclusion into high-throughput genotyping platforms for clinical diagnostic services, current systems are very limited. Our approach provides a simple and inexpensive method for the

Elisabet Sjöberg Wester, Julia Gustafsson, Beverly Snell, Peggy Spruell, Åsa Hellberg, Martin L. Olsson, Jill R. Storry

Immunohematology, Volume 25 , ISSUE 4, 165–169

Article | 10-April-2021

An automated approach to determine antibody endpoint titers for COVID-19 by an enzyme-linked immunosorbent assay

directed against the RBD correlate with neutralizing antibody levels, demonstrating that anti-RBD levels may provide a useful surrogate for SARS-CoV-2 antibody activity.14 This finding is especially important when considering that, although neutralizing assays are often considered the gold standard for assessing antiviral antibody activity, these approaches can be difficult to establish as high-throughput tests, making it difficult to implement this approach clinically.15 Using an enzyme-linked

A.D. Ho, H. Verkerke, J.W. Allen, B.J. Saeedi, D. Boyer, J. Owens, S. Shin, M. Horwath, K. Patel, A. Paul, S.-C. Wu, S. Chonat, P. Zerra, C. Lough, J.D. Roback, A. Neish, C.D. Josephson, C.M. Arthur, S.R. Stowell

Immunohematology, Volume 37 , ISSUE 1, 33–43

research-article | 30-November-2020

Performance evaluation of vertical handover in Internet of Vehicles

enhanced Access Network Discovery and Selection Function (ANDSF) was presented to perform a BS selection in the network. This algorithm combines with multilayer perceptron (MLP). The parameters were load, signal strength, throughput, and delay. The traditional workflow of the ANDSF is illustrated in Figure 1. Figure 1: Workflow procedure of ANDSF (Ndashimye et al., 2020). The ANDSF was equipped within the EPC which was started to be used in 3G and also on advanced radio access networks. This

Shaik Mazhar Hussain, Kamaludin Mohamad Yusof, Shaik Ashfaq Hussain, Rolito Asuncion

International Journal on Smart Sensing and Intelligent Systems, Volume 14 , ISSUE 1, 1–16

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