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  • Journal Of Ultrasonography

 

research-article | 31-December-2019

The use of high-frequency ultrasonography in the assessment of selected female reproductive structures: the vulva, vagina and cervix

ultrasonography (HFUS) of the vulva, vagina and cervix was performed. Equipment Two high-frequency scanners were used in the study: Episcan (Longport International, Ltd., USA) with a mechanical transducer of 50 MHz and DermaView (Dramiński S.A., Poland) with a mechanical transducer of 48 MHz. Due to the shape and structure of both transducers, the transducers from both of the Episcan and DermaView machines were used for vulvar examination, while vaginal structures and the cervix were evaluated using the

Marian Stanisław Migda, Michał Migda, Rafał Słapa, Robert Krzysztof Mlosek, Bartosz Migda

Journal of Ultrasonography, Volume 19 , ISSUE 79, 261–268

case-report | 31-December-2019

The utility of high-frequency ultrasonography in preoperative assessment of vulvar Paget’s disease – a case report

Introduction Paget’s disease of the vulva (PDV) is a rare superficial skin cancer accounting for less than 1% of all vulvar neoplasias(1). Extramammary manifestation occurs in the apocrine glands and is a form of intraepidermal adenocarcinoma(2,3). The most commonly affected regions are the vulva, perineum, perianal region, scrotum, penis, or pubic area(4). Although surgery is the most effective treatment, a complete excision is challenging due to PDV intraepithelial reticular spread(2

Michał Migda, Marian Stanisław Migda, Bartosz Migda, Robert Krzysztof Mlosek, Marek Maleńczyk

Journal of Ultrasonography, Volume 19 , ISSUE 79, 311–313

research-article | 31-December-2019

The usefulness of high-frequency ultrasonography in the evaluation of vulvar dermatoses in postmenopausal women – a preliminary report

Introduction The vulva is an external female genital organ, which has a complex anatomy composed of hymen, anal margin, labia minora, labia majora and clitorial hood(1). Different types of epithelium predispose to a wide range of conditions known as vulvar dermatoses(2,3). Vulvovaginal tissues are particularly susceptible to many irritants and allergens compared to other skin sites(4). Tissue atrophy and immunologic changes that occur with aging increase the risk of the most common vulvar

Michał Migda, Marian Stanisław Migda, Bartosz Migda, Marek Maleńczyk

Journal of Ultrasonography, Volume 19 , ISSUE 79, 282–286

research-article | 31-December-2019

The usefulness of high-frequency ultrasonography in the preoperative evaluation of vulvar cancer – a case series

. All patients were over 70 years old. The main complain was pain and problems with urination (case 3) caused by urethral infiltration. First symptoms appeared approximately a year before in the form of a small red dot with itching. In gynecological examination, case 1 and 2 were solid tumors in the labia majora, with edema in the surrounding tissues. Palpation was difficult due to severe pain. Case 3 presented with massive edema of the vulva and a solid tumor infiltrating vagina and penetrating to

Michał Migda, Marian Stanisław Migda, Bartosz Migda, Marek Maleńczyk

Journal of Ultrasonography, Volume 19 , ISSUE 79, 305–310

research-article | 30-November-2020

Muscles of the male and female copulatory organs of Bursaphelenchus mucronatus and Chiloplacus sp. (Nematoda: Rhabditida)

). (A–C) Series of lateral views of the vulval region at different optical levels. (B) Vulval flap looks flap-shaped with its ending not reaching the body wall (arrow). (A, C) Vulval flap is fold-shaped at the margins of the vulva (arrow): the fold continues to the body wall. Abbreviations: lip—posterior vulval lip; vf—vulval flap; vg—vagina; vo—vulval opening. (Scale bars: A–D = 30 µm; E–G = 50 µm). Figure 2: Mating of Bursaphelenchus mucronatus (from orig. video). (A) Arrangement of the male

Alexander Ryss, Anatoly A. Petrov

Journal of Nematology, Volume 53 , 1–21

research-article | 30-November-2020

Discopersicus hexagrammatus n. sp. (Rhabditida: Tylenchidae), the second species of the genus

(conus/stylet %) 35 33.6 ± 1.1 (32-35) 3.4 33.1 ± 0.5 (33-34) 1.6 Anterior end to valve of median bulb 39 42.0 ± 2.2 (39-45) 5.4 40.8 ± 2.7 (38-44) 6.8 (Anterior end to pharyngeal intestinal junction) Pharynx length 108 111.0 ± 4.0 (105-117) 3.6 111.0 ± 3.5 (107-115) 3.1 M.B. 36 37.8 ± 0.9 (36-39) 2.4 36.7 ± 1.3 (35-39) 3.8 Anterior end to excretory pore 89 88.2 ± 1.8 (85-90) 2.0 86.6 ± 1.6 (84-88) 1.9 Anterior end to vulva 470 455.0 ± 22.5 (408-485) 4.9 – – Anterior end to anus 641 620.0

Manouchehr Hosseinvand, Ali Eskandari, Joaquín Abolafia, Reza Ghaderi

journal of Nematology, Volume 53 , 1–10

research-article | 30-November-2019

Oostenbrinkia pedrami n. sp. (Dorylaimida: Aulolaimoididae) from Iran, with molecular phylogenetic relationships to some other Dorylaimida Pearse, 1942

). (A): Neck region; (B, D, E): Anterior body region in female; (C) Anterior body region; (F, G): Posterior body region; (H, I): Vulva region; (J): Part of the reproductive system showing reflexed ovaries; (K-M): Pharyngeal bulb with variation of triquetrous chamber in its lumen. (Scale bars = 10 µm). Measurements Measurements of the new species are given in Table 1. Table 1. Morphometrics of Oostenbrinkia pedrami n. sp. Character Holotype Paratypes n – 10 L 847 747 ± 83

Farahnaz Jahanshahi Afshar

Journal of Nematology, Volume 52 , 1–7

research-article | 30-November-2019

Aphelenchus yinyuensis n. sp. (Tylenchina: Aphelenchoididae) found in Terminalia sp. in China

. Oviduct is comparatively longer, connecting ovary and spermatheca. Spermatheca is round or oblong, containing round sperm, present in majority of specimens. Uterus is thick-walled. Vagina wall is parallel not sclerotised, vulva pore shape, and lips simple without flap. In some specimens, vulval region appears sunken. PUS is well developed, extending for ca 44.5 to 82.7% of vulva to anus distance. Rectum and anus are visible. Tail is straight, cylindrical, ca 2.7 times longer than anal body diam. Tail

Gu Jianfeng, Munawar Maria, Yiwu Fang, Liu Lele, Xianfeng Chen, Bo Cai

Journal of Nematology, Volume 52 , 1–12

Research Article | 31-May-2018

Postembryonic Ventral Nerve Cord Development and Gonad Migration in Steinernema carpocapsae

Hung Xuan Bui, Nathan E. Schroeder

Journal of Nematology, Volume 50 , ISSUE 1, 27–32

research-article | 24-April-2019

Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae)

key can be freely accessed at http://nematodeidentification.mypressonline.com/category/identification-tool/. Results SYSTEMATICS Rotylenchus rhomboides n. sp. Figures 1–6, Table 1–3. Figure 1 Rotylenchus rhomboids n. sp., (drawings ♀). (A) entire body; (B) en face view; (C) head region; (D) esophageal region; (E) lateral field at vulva region; (F) vulva region; (G) tail region; (H) lateral field at tail region. (B, C based on SEM illustrations) (A, E, F, G, H: Holotype) (Scale bar: A: 25 µm

Huu Tien Nguyen, Quang Phap Trinh, Marjolein Couvreur, Phougeishangbam Rolish Singh, Wilfrida Decraemer, Wim Bert

Journal of Nematology, Volume 51 , 1–20

Article | 04-December-2017

Morphological and Molecular Characteristics of Pratylenchus haiduongensis sp. n., a New Species of Root–Lesion Nematodes Associated with Carrot in Vietnam

region. Sometimes the appearances of oblique broken striaes divide the lateral field into five or six incisures. The ovary is distinct with one row of oocytes. Spermatheca is oval in shape with round central cavity, without sperm or reduced in some specimens. The postvuval uterine sac is long surpassing the vulva body diameter by 2 to 2.5 times (PUS = 31 to 65 mm). High vulva position with V = 66 to 75%. The tail shape can be subhemispherical with a smooth, slightly indented, broadly smooth, or cleft

THI DUYEN NGUYEN, THI MAI LINH LE, HUU TIEN NGUYEN, THI ANH DUONG NGUYEN, GRACIA LIEBANAS, QUANG PHAP TRINH

Journal of Nematology, Volume 49 , ISSUE 3, 276–285

research-article | 18-March-2020

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

nothos meaning false, krikos meaning ring, nema meaning nematodes, and oides meaning shape (Siddiqi, 2000). The important diagnostic characteristics of this genus include body annuli with fine longitudinal striae making margins that look finely crenated; the second cephalic annulus of female is offset and collar like. Lips have four distinct submedian lobes. Vulva is closed, and anterior lip overhanging in type species. Tail is conoid tapering to acute or sub-acute terminus. Juveniles have crenate

Munawar Maria, Wentao Miao, Ruihang Cai, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–14

research-article | 06-November-2020

Morphological and molecular characterization of Epidorylaimus procerus sp. n. (Dorylaimida: Qudsianematidae) from Vietnam

length 32 33.4 ± 1.2 (32-35) Odontophore length 32 30.2 ± 1.5 (28-33) Neck length 432 435 ± 18 (415-461) Pharyngeal expansion length 204 212 ± 13 (194-238) Body diam. at neck base 61 65.0 ± 4.0 (61-73)  Mid-body 77 72.6 ± 3.4 (68-79)    Anus/cloaca 36 35.2 ± 1.3 (32-36) Distance vulva – anterior end 982 938 ± 43 (885-1012) Prerectum length 91 91.8 ± 18.4 (70-123) Rectum length 42 42.5 ± 4.6 (35-49) Tail length 182 171 ± 9.2 (157-186) Note: Measurements are in μm, except L

Thi Anh Duong Nguyen, Reyes Peña-Santiago

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2018

Cryphodera gayae n. sp. (Nematoda: Heteroderidae), from root of Lagerstroemia indica in Korea

) 286.5 166.6–329.1 259.3 ± 46.2 Neck 82.5 77.2–164.8 103.6 ± 26.9 L (inc. neck)/W ratio 1.6 1.6–2.2 1.8 ± 0.2 Stylet length 30.7 28.2–31.9 30.1 ± 1.0 Anterior end to median bulb 65.0 53.9–93.7 70.3 ± 14.7 Length of medium bulb 28.3 22.6–31.9 25.9 ± 2.9 Width of medium bulb 25.2 20.6–29.9 24.3 ± 3.2 Vulva-nus distance 46.9 35.8–51.6 44.7 ± 4.3 Vulval slit length – 51.9–54.1 52.6 ± 1.3 Excretory pore from anterior end 80.1 72.6–124.5 92.4 ± 17.5 Second stage (J2) n

Heonil Kang, Jongmin Seo, Garam Han, Donggeun Kim, Insoo Choi

Journal of Nematology, Volume 51 , 1–12

research-article | 03-June-2019

Morphological and molecular characterization of Labronema montanum sp. n. (Dorylaimida, Dorylaimidae) from Spain

; B–D, H–M = 10 µm; F, G = 50 µm.) Figure 3: Labronema montanum sp. n. from Spain (male, LM). (A): Entire. (B–D): Pharyngo-intestinal junction showing the three lobes. (E): Posterior body region. (F): Spicule. (G, H): Male. (I): Lateral guiding piece. (J): Sperm cells. (Scale bars: A = 500 µm; D–B = 20 µm; E = 50 µm; F–H = 10 µm; I = 5 µm; J = 10 µm). Figure 4: Labronema montanum sp. n. from Spain (SEM). (A): Lip region in ventral view. (B): Same in frontal view. (C): Vulva, ventral

R. Peña-Santiago, J. Abolafia

Journal of Nematology, Volume 51 , 1–11

research-article | 30-November-2018

First report of Longidorus mindanaoensis Coomans, De Ley, Jimenez and De Ley, 2012 (Nematoda: Longidoridae) From a Mangrove Forest in Vietnam

original description of the species by Coomans et al. (2012). Some unique morphological and morphometric characters like the shape of lip region and amphidial fovea, the characters of the esophageal bulb (its small size and shape as well as the arrangement of glands nuclei) and the position of vulva delimiting the species well corroborated the identity of the species. However, new morphometric data ranges were recorded for the species as follows: the females and males of the presently studied

Thi Duyen Nguyen, Huu Tien Nguyen, Thi Mai Linh Le, Neriza Nobleza, Quang Phap Trinh

journal of nematology, Volume 51 , 1–5

Research Article | 03-September-2018

Nothotylenchus andrassy n. sp. (Nematoda: Anguinidae) from Northern Iran

Nothotylenchus andrassy n. sp. is described and illustrated from moss (Sphagnum sp.) based on morphology and molecular analyses. Morphologically, this new species is characterized by a medium body size, six incisures in the lateral fields, and a delicate stylet (8–9 µm long) with clearly defined knobs. Pharynx with fusiform, valveless, non-muscular and sometimes indistinct median bulb. Basal pharyngeal bulb elongated and offset from the intestine; a long post-vulval uterine sac (55% of vulva to

Parisa Jalalinasab, Mohsen Nassaj Hosseini, Ramin Heydari

Journal of Nematology, Volume 50 , ISSUE 2, 219–228

research-article | 30-November-2018

Morphological and Molecular Characterization of Oscheius saproxylicus sp. n. (Rhabditida, Rhabditidae) From Decaying Wood in Spain, With New Insights into the Phylogeny of the Genus and a Revision of its Taxonomy

diameter at neck base 22 23.9 ± 1.7 (20–28) Body diameter at midbody 22 28.5 ± 3.4 (21–37) Vulva - anterior end 469 459 ± 49.0 (366–549) Rectum length 48 44.8 ± 3.8 (40–54) Anal body diameter 13 13.9 ± 1.1 (11–16) Tail length 72 76.3 ± 5.0 (67–88) Phasmid - anus distance 19 23.9 ± 3.4 (20–30) Notes: Demanian indices (de Man, 1880): a = body length/body diameter; b = body length/pharynx length; c = body length/tail length; c’ = tail length/anal body diameter; V = (distance from

Joaquín Abolafia, Reyes Peña-Santiago

journal of nematology, Volume 51 , 1–21

research-article | 14-December-2020

Morphological, morphometrical, and molecular characterization of Metarhabditis amsactae (Ali, Pervez, Andrabi, Sharma and Verma, 2011) Sudhaus, 2011 (Rhabditida, Rhabditidae) from India and proposal of Metarhabditis longicaudata as a junior synonym of M. amsactae

Testis 260 ± 38.2 (192-372) 198 ± 15 (188-222) – Posterior genital branch 278 ± 27.5 (229-321) – – Vagina length 24.5 ± 4.1 (17-30) – – Vulva–anterior end (VA) 480 ± 64 (380-579) – – Rectum length 31 ± 6.4 (22-42) – 15.0 ± 4.0 (9-23) Anal body width (ABW) 22 ± 2.7 (16-28) 20.9 ± 2.5 (17-27) 11.2 ± 2.2 (9-17) Tail length (T) 87 ± 10.5 (68-101) 63 ± 8.2 (49-62) 52 ± 5.9 (48-58) Spicules length – 41 ± 7.5 (34-49) – Gubernaculum length – 19.6 ± 3.5 (16-28) – Notes: = Character

Aashaq Hussain Bhat, Shreyansh Srivastava, Aasha Rana, Ashok Kumar Chaubey, Ricardo A. R. Machado, Joaquín Abolafia

Journal of Nematology, Volume 52 , 1–23

Article | 04-December-2017

A New Species of the Rare Genus Anguillonema Fuchs, 1938 (Nematoda: Hexatylina, Sphaerularioidea) with Its Molecular Phylogenetic Study

Abstract: Anguillonema amolensis n. sp. is described and illustrated based on its morphological, morphometric, and molecular characters. The new species is characterized by its 575 to 820 mm long and wide body (body width at vulva = 30 to 59 mm), irregularly ventrally curved after fixation, five to six lines in lateral fields, 6.0 to 7.5 mm long stylet with small rounded knobs, pharynx lacking median bulb, pharyngo-intestinal junction anterior to nerve ring and excretory pore, females with

MAHYAR MOBASSERI, MAJID PEDRAM, EBRAHIM POURJAM

Journal of Nematology, Volume 49 , ISSUE 3, 286–294

Research Article | 31-May-2018

Delatylus andersoni n. gen., n. sp. (Nematoda: Neotylenchidae) Isolated from White Pine (Pinus monticola) Lumber from USA and Intercepted in Ningbo, China

opening large, and relative proximity of vulva to the anus. Detailed morphological and molecular characterization of the new genus/species is presented along with the comparison of the related genera.

Qing Yu, Maria Munawar, Jianfeng Gu, Weimin Ye

Journal of Nematology, Volume 50 , ISSUE 1, 69–76

Research Article | 17-October-2018

Description of Xiphinema parachambersi sp. n. (Nematoda: Longidoridae) from Imported Ornamental Plants in Japan with a Key to Xiphinema Species in Group 1

Xiphinema parachambersi n. sp. recovered in Ningbo, China, from the rhizosphere of ornamental plants (Gardenia jasminoides and Euonymus hamiltonianus) imported from Japan is described. The new species is characterized by a long female body 1,830 to 2,109 μm long, odontostyle 105 to 116 μm long, reproductive system mono-opisthodelphic, vulva located anteriorly at 25.2 to 27.7% of total body, long ovary 119 to 292 μm with simple uterus and Z-organ absent, female tail elongated conoid with a

Munawar Maria, Weimin Ye, Qing Yu, Jianfeng Gu

Journal of Nematology, Volume 50 , ISSUE 3, 369–386

research-article | 25-May-2020

Description and molecular phylogeny of Mesocriconema abolafiai n. sp. (Nematoda: Criconematidae) from Iran

Siddiqi (2000) still considered Macroposthonia as a valid name. Brzeski et al. (2002) accepted this synonymy and provided a compendium of the genus Mesocriconema with 90 species (species having open vulva and submedian lobes arising from reduced pseudolips). Moreover, they considered that Mesocriconema differs from Criconemoides (species with closed vulva and pseudolips not reduced). Geraert (2010) replaced some species in the genus Neobakernema (Ebsary, 1981b) by validation of this genus and listed

Hossein Mirbabaei Karani, Ali Eskandari, Reza Ghaderi, Akbar Karegar

Journal of Nematology, Volume 52 , 1–17

research-article | 30-November-2020

Morphological and molecular characterization of Xiphinemella esseri Chitwood, 1957 (Dorylaimida: Leptonchidae) from Florida, with the first molecular study of the genus

, and a wider proximal portion; its length 194 to 296 μm long or 3.6 to 5.9 times the corresponding body diameter; almost always containing sperm cells. Uterine eggs ovoid, 180 × 55 μm (n = 1), 3.3 times as long as wide. Vagina extending inwards 26 to 31 μm or 47 to 57% of corresponding body diameter, pars proximalis 17–23 × 14–18 μm, with sigmoid walls and surrounded by weak musculature, pars refringens absent, pars distalis well-developed 5 to 9 μm long. Vulva pre-equatorial to equatorial

Sergio Álvarez-Ortega, Sergei A. Subbotin, Renato N. Inserra

Journal of Nematology, Volume 53 , 1–9

research-article | 30-November-2020

Morphological and molecular characterization of Paratylenchus beltsvillensis n. sp. (Tylenchida: Paratylenchidae) from the rhizosphere of pine tree (Pinus virginiana Mill) in Maryland, USA

region with vulva, uterus, and spermatheca; C: Female lip region with stylet; D: Female specimen with vulval opening; E: Lateral field (mid-body); F: Male specimen with spicules and gubernaculum; G–I: Female tails with vulval opening and tail variations; J: Male tale showing spicules and gubernaculum. Figure 2: Photomicrographs of Paratylenchus beltsvillensis n. sp. A–E: Female anterior ends; F: Male anterior end; G–J: Female posterior ends with vulva area (arrows) and tails; K: Lateral field

Mihail R. Kantor, Zafar A. Handoo, Sergei A. Subbotin, Gary R. Bauchan, Joseph D. Mowery

Journal of Nematology, Volume 53 , 1–10

research-article | 22-February-2021

Rotylenchus wimbii n. sp. (Nematoda: Hoplolaimidae) associated with finger millet in Kenya

wimbii n. sp. specimens mounted in glycerin. Female character n 19 Body length (L) 690  ±  44 (620-760) a = L/MBD 27.3 ± 3.0 (23.0-33.0) b = L/Anterior end to pharynx-intestine junction 5.8 ± 0.3 (5.0-6.0) b′ = L/Anterior end to end of pharyngeal gland 5.1 ± 0.4 (4.5-6.1) c = L/Tail length 66.0 ± 12.0 (49.0-91.0) c′ = Tail length/ABD 0.6 ± 0.1 (0.3-0.8) V = Anterior end to vulva opening/L × 100 58 ± 1 (55-60) Lip region height 4.6 ± 0.3 (4.2-5.3) Lip region

Phougeishangbam Rolish Singh, Gerrit Karssen, Kelvin Gitau, Cecilia Wanjau, Marjolein Couvreur, Njira Njira Pili, Godelieve Gheysen, Wim Bert

Journal of Nematology, Volume 53 , 1–14

research-article | 30-November-2020

Morphological and molecular characterization of Iotonchus lotilabiatus n. sp. (Nematoda: Iotonchidae) from Lao Cai Province, Vietnam

cloacal opening to posteriormost supplement – – 20.5-23 (21.4 ± 1.0) Distance from cloacal opening to anterior most supplement – – 201-241.5 (218.9 ± 15.8) Tail length 572 694-974 (818 ± 125.3) 500-798 (623 ± 114) Notes: All measurements are in µm except where indicated. a  =  body length/maximum body width; b = body length/pharyngeal length; c = body length/tail length; c′ = tail length/body width at anus; V =  (distance from anterior end to vulva/body length)x100. L = body length

Tam T. T. Vu, Thi Mai Linh Le, Thi Duyen Nguyen

Journal of Nematology, Volume 53 , 1–22

Research Article | 03-September-2018

Morphological Re-Description and 18 S rDNA Sequence Confirmation of the Pinworm Aspiculuris tetraptera (Nematoda, Heteroxynematidae) Infecting the Laboratory Mice Mus musculus

developed labial papillae and one pair of amphidial pores. Esophagus divided into cylindrical corpus and globular bulb. Distinct cervical alae interrupted at the level of esophago–intestinal junction forming an acute angle. At the caudal end, twelve caudal papillae in male worms while an ovijector apparatus opening and a vulva surrounded by protruded lips in females were observed. The general morphological criteria include this nematode with other Aspiculuris species which were compared in the present

Rewaida Abdel-Gaber, Fathy Abdel-Ghaffar, Saleh Al Quraishy, Kareem Morsy, Rehab Saleh, Heinz Mehlhorn

Journal of Nematology, Volume 50 , ISSUE 2, 117–132

research-article | 30-November-2020

Description of Longidorella (Saevadorella) caspica n. sp. (Dorylaimida: Nordiidae) from north Iran

-G: Female; D: Male. A: Pharynx; B: Anterior body region; C: Anterior end; D: Posterior body region; E and G: Tail and posterior body region, F: Posterior genital tract. Figure 2: Photomicrographs of Longidorella (Saevadorella) caspica n. sp. A-D, F-I: Female; E: Male. A-D: Anterior and cephalic region; E and F: Entire body; G: Vulva in ventral view; H: Mature egg inside female reproductive system; I: Sperm inside the uterus. (Scale bars = 10 μm, except E and F = 100 μm). Figure 3

Fariba Heydari, Mohammad Reza Atighi, Ebrahim Pourjam, Majid Pedram

Journal of Nematology, Volume 53 , 1–11

research-article | 30-November-2020

Molecular and morphological characterization of a first report of Cactodera torreyanae Cid del Prado Vera & Subbotin, 2014 (Nematoda: Heteroderidae) from Minnesota, the United States of America

population of C. torreyanae was also used for molecular study. Figure 1: Photomicrographs of cysts, vulva cones and second-stage juveniles (J2) of Cactodera torreyanae. A: Entire cyst; B-C: Vulva cones; D-E: Anterior end of J2s; F: Cyst anterior end (arrow showing anterior end of J2 with stylet sticking out); G-H: Tails of J2s; I: Lateral field with 4 incisures for J2. Molecular study DNA was isolated from single juveniles disrupted in 20 µl Nematode Extraction Buffer. DNA extraction

Zafar A. Handoo, Andrea M. Skantar, Sergei A. Subbotin, Mihail R. Kantor, Maria N. Hult, Michelle Grabowski

Journal of Nematology, Volume 53 , 1–5

research-article | 30-November-2019

Hemicycliophora ahvasiensis n. sp. (Nematoda: Hemicycliophoridae), and data on a known species, from Iran

–180) 168 Diam. at mid-body 39 38.4 ± 3.6 (32–46) 33 Diam. at anus (ABD) 27 26.8 ± 1.7 (24–29) 23 Diam. at vulva 38 38.4 ± 2.1 (35–43) – Vulva-anterior body distance 744 700 ± 43 (653–751) – Vulva-tail terminus distance 125 129.5 ± 6.0 (113–142) – Spermatheca-vulva distance 89 87.6 ± 13.2 (74–121) – Lip diam. 15 15.7 ± 0.9 (14–18) 14 Lip height 7 6.7 ± 0.7 (6–9) 6 First body annulus diam. 16 16.9 ± 0.9 (15–19) 15 Second body annulus diam. 18 18.6 ± 1.1 (16–21) 16 Pharynx

Sedighe Azimi, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 52 , 1–19

research-article | 01-October-2021

Redescription and phylogenetic analysis of the type species of the genus Panagrellus Thorne, 1938 (Rhabditida, Panagrolaimidae), P. pycnus Thorne, 1938, including the first SEM study

) Anterior oviduct length 56.1 ± 15.8 (40–91) – Anterior uterus length 377.4 ± 63.8 (287–496) – Post-vulval uterine sac length 133.4 ± 22.2 (99–162) – Vagina length 42.0 ± 8.5 (36–48) – Vulva – anterior end 740.9 ± 74.0 (668–863) – Rectum or cloaca length 28.2 ± 3.8 (20–32) 9.8 ± 0.7 (9–11) Anal body diameter 25.1 ± 2.8 (22–30) 27.2 ± 3.4 (23–34) Tail length 148.9 ± 11.9 (133–170) 119.6 ± 10.1 (104–137) Phasmid - anus distance 55.6 ± 6.1 (46–67) 47.7 ± 6.7 (36–57) Spicules length (arc

Joaquín Abolafia, Matteo Vecchi

Journal of Nematology, Volume 53 , 1–20

Research Article | 26-September-2018

Xiphinema japonicum n. sp. (Nematoda: Longidorinae) from the Rhizosphere of Japanese Podocarpus macrophyllus (Thunb.), a Cryptic Species Related to Xiphinema bakeri Williams, 1961

Xiphinema japonicum n. sp., isolated in Ningbo, China, from the rhizosphere of Podocarpus macrophyllus (Thunb.) imported from Japan is described. The new species belongs to Xiphinema non-americanum group 7 and is characterized by medium body length (3.0–3.7 mm), total stylet length 190–201 μm, vulva located anteriorly (V = 30.5%–35.3%), two equally developed female genital branches without uterine differentiation (no Z or pseudo-Z organ and/or spines in the uteri), short tail, convex-conoid

LIRONG ZHAO, WEIMIN YE, MUNAWAR MARIA, MAJID PEDRAM, JIANFENG GU

Journal of Nematology, Volume 49 , ISSUE 4, 404–417

research-article | 16-April-2019

Description of a new dagger nematode, Xiphinema barooghii n. sp. (Nematoda: Longidoridae) and additional data on the three known species of the genus from northwest of Iran

, spindle-shaped and scattered between the enlarged distal portion and the ovejector, with a lack of sperm in the genital tract. Ovejector is well developed (71–84 × 15–28 μm); vagina is perpendicular to body axis, 31–37 μm long or 42–52% of corresponding body diameter in lateral view and surrounded by robust muscles. Vulva, a transverse slit, pre-equatorial in position, is present. Pre-rectum variable is 503‒700 μm in length and the rectum length is 0.7‒1.1 times anal body diameter. A short tail

Nasir Vazifeh, Gholamreza Niknam, Habibeh Jabbari, Arezoo Naghavi

Journal of Nematology, Volume 51 , 1–17

research-article | 16-April-2020

A new stunt nematode, Geocenamus chengi n. sp. (Nematoda: Merliniinae) in the rhizosphere of tea (Camellia sinensis) from Zhejiang Province, China

: K-M: male tails (Scale bars = A; D-M = 10 μm, B,C = 5 μm). Figure 2: Light photomicrographs of Geocenamus chengi n. sp. Female. A: entire body; B: lateral lines; C, D: pharyngeal regions, arrow pointing on the excretory pore (exp): E: gonad; F: vulval region arrows pointing on vulva (v) and spermatheca (sp); G, H: vulval region; I-K: female tails arrows pointing on anus (a) and phasmid (ph) (Scale bars = A = 100 μm; B-K = 10 μm). Figure 3: Light photomicrographs of Geocenamus chengi n

Munawar Maria, Wentao Miao, Pablo Castillo, Jingwu Zheng

Journal of Nematology, Volume 52 , 1–13

research-article | 30-November-2019

Oscheius myriophila (Nematoda: Rhabditida) isolated in sugar cane soils in Mexico with potential to be used as entomopathogenic nematode

slightly narrow. Nerve rings usually surround the mid portion of the isthmus. Excretory pores are conspicuously located at the level of the basal bulb. The excretion duct is circularized and curved anteriorly, then posteriorly. Vulva near the mid body and lips protruding. Male: Similar to the female in general morphology except for a smaller size of 720 to 910 µm in length. The body is straight after fixation. Spicules are paired, separate, symmetrical, and slightly curved ventrally with hooked tips

Iveth del Rocio Castro-Ortega, Juan Manuel Caspeta-Mandujano, Ramón Suárez-Rodríguez, Guadalupe Peña-Chora, José Augusto Ramírez-Trujillo, Karina Cruz-Pérez, Iván Arenas Sosa, Víctor Manuel Hernández–Velázquez

Journal of Nematology, Volume 52 , 1–8

research-article | 30-November-2019

A proposal of Bursaphelenchus uncispicularis Zhuo, Li, Li, Yu & Liao, 2007 as a junior synonyms of B. yongensis Gu, Braasch, Burgermeister, Brandstetter & Zhang, 2006

Bursaphelenchus yongensis Gu, Braasch, Burgermeister, Brandstetter & Zhang, 2006 (Gu et al., 2006) was originally described from Pinus massoniana Lamb. in Ningbo city, Zhejiang province, China. It is characterized by a relatively slim body (a = 42 and 57 for females and males, respectively), excretory pore located at level of median bulb, lateral field with three lines, small vulval flap present, long post-uterine branch extending 2/3 to 3/4 of the vulva to anus distance and a conoid female

Jianfeng Gu, Kan Zhuo, Jinling Liao

Journal of Nematology, Volume 52 , 1–3

research-article | 30-November-2020

Laimaphelenchus africanus n. sp. (Tylenchomorpha: Aphelenchoididae) from South Africa, a morphological and molecular phylogenetic study, with an update to the diagnostics of the genus

region; (L): Spicule. Figure 2: Light photomicrographs of Laimaphelenchus africanus n. sp. (A-C, E, G-K, female; D, F, F1, F2, male). (A): Part of pharynx; (B-D): Anterior end; (E): Part of reproductive system; (G and H): Vulva; (F, I-K): Posterior body region; (F1): Spicule; (F2, I1-K1): Details of the stalk at the tail tip. (Scale bars: B-D, F1, F2, G, H, I1-K1 = 5 µm; A, E, F, I-K = 10 µm). Figure 3: Scanning electronic microscopy of Laimaphelenchus africanus n. sp., female. (A-C

Farahnaz Jahanshahi Afshar, Milad Rashidifard, Joaquín Abolafia, Miloslav Zouhar, Hendrika Fourie, Majid Pedram

Journal Of Nematology, Volume 53 , 1–14

Article | 21-July-2017

Molecular and Morphological Characterization of Xiphinema chambersi Population from Live Oak in Jekyll Island, Georgia, with Comments on Morphometric Variations

rounded and set off from head; total stylet length 170 to 193 mm; vulva at 20.4% to 21.8% of body length; a monodelphic, posterior reproductive system; elongate, conoid tail with a blunt terminus and four pairs of caudal pores, of which two pairs are subdorsal and two subventral. Sequence data from the D2–D3 region of the 28S rRNA molecule subjected to GenBank sequence comparison using BLAST showed that the sequence had 96% and 99% similarity with X. chambersi from Alabama

ZAFAR A. HANDOO, LYNN K. CARTA, ANDREA M. SKANTAR, SERGEI A. SUBBOTIN, STEPHEN W. FRAEDRICH

Journal of Nematology, Volume 48 , ISSUE 1, 20–27

Research Article | 03-September-2018

Description of Longidorus azarbaijanensis n. sp. (Dorylaimida: Longidoridae) from Iran

long odontostyle and odontophore, respectively, 95.0 to 113.5 µm long pharyngeal bulb, didelphic-amphidelphic reproductive system with long tubular uteri lacking sperm cells, vulva located at 52.0% to 56.4%, conical tail dorsally convex, ventrally flat, with bluntly rounded wide tip, for juvenile developmental stages and absence of males. The general body shape of the new species is similar to that of five known species of the genus namely L. euonymus, L. perangustus, L. persicus, L. protae and L

Farshad Gharibzadeh, Ebrahim Pourjam, Majid Pedram

Journal of Nematology, Volume 50 , ISSUE 2, 207–218

research-article | 30-November-2019

Intraspecific variation in phenotypic and phylogenetic features among Pratylenchus penetrans isolates from Wisconsin, USA

multiple species keys for the genus Pratylenchus (Loof, 1960; Corbett, 1970; Handoo and Golden, 1989; Ryss, 2002; Castillo and Vovlas, 2007). Consensus morphological characters are three lip annuli, round spermatheca, presence of males, smooth tail tip, and four incisures in the lateral field. Morphological measurements and indices of morphological features are also important, particularly the proportional position of the vulva (V%) and relative length of the esophagus and tail. Morphological features

Kanan Saikai, Ann E. MacGuidwin

Journal of Nematology, Volume 52 , 1–17

research-article | 01-April-2021

First record of Oscheius myriophilus (Poinar, 1986) (Rhabditida: Rhabditidae) from Iran; and its efficacy against two economic forest trees pests, Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) and Hyphantria cunea (Drury, 1773) (Lepidoptera: Erebidae) in laboratory condition

to vulva/L)×100 – 49.9 ± 2.0 – – – – – – – – (45.5–53.1) – – – – – – – Stoma length 17.5 ± 0.5 17.6 ± 0.8 16.1 ± 1.4 17 20 – – – – (17–18) (17–20) (14–20) (16–19) (18–21) – – (18–21) – Stoma width 3.4 ± 0.5 4.2 ± 0.2 2.7 ± 0.3 3.2 3.2 – – – – (3–4) (4–4.5) (2.5–4) – – – – <4.5 – Pharynx length 196 ± 9.7 176.3 ± 17.1 134.5 ± 7.1 187 185 129 – – 134.6 ± 3.2 (180–208) (144–200) (125–145) (161–200) (174–193) (120–136) – – (128.8–139.8) Max. body diam. 66.8 ± 11.5 52.3 ± 6.8 27.8

Reihaneh Gholami Ghavamabad, Ali Asghar Talebi, Mohammad Mehrabadi, Mohammad Ebrahim Farashiani, Majid Pedram

Journal of Nematology, Volume 53 , 1–16

research-article | 30-November-2020

An interesting rare tylenchid species, Antarctenchus urmiensis n. sp. (Tylenchomorpha; Psilenchidae) from Urmia Lake islands, northwest Iran, with a discussion on the taxonomy of related genera

tail; (N) Bursa; (O&P) Entire body; (Q) Vulval region; (R) Spicule and gubernaculum. (Scale bars: A-N, Q&R = 10 μm; O&P = 50 μm). Figure 3: Scanning electron microscopic (SEM) images of Antarctenchus urmiensis n. sp. (Female) (A-C) Anterior end in ventral, sublateral and frontal views, respectively (arrows pointing the amphidial openings); (D) Deirid (withe arrow) and excretory pore in lateral view (black arrow); (E) Secretory-excretory pore (arrow); (F) Lateral field; (G&H) Vulva in lateral

Mohammad Amiri Bonab, Joaquín Abolafia, Majid Pedram

Journal of Nematology, Volume 53 , 1–14

Article | 21-July-2017

First Report of Longidorus kuiperi and Rotylenchus eximius from Coastal Sand Dunes in Crete, Greece

Longidorus kuiperi Brinkman, Loof and Barbez, 1987, and Rotylenchus eximius Siddiqi, 1964. The morphology of L. kuiperi females (six specimens studied) was characterized by having a slender body; very broad lip region (27 6 1.5 [25 to 30] mm in width); short, hemispherical tail; body length of (7.1 6 0.8 [6.5 to 8.5] mm); vulva position at 47% to 55% of body length; odontostyle length of (105 6 6.5 [90 to 115] mm); males very common (but less frequent than females [45% vs 55%]); tail region with 15 to 20

EMMANUEL A. TZORTZAKAKIS, CAROLINA CANTALAPIEDRA-NAVARRETE, ANTONIO ARCHIDONA-YUSTE, JUAN E. PALOMARES-RIUS, PABLO CASTILLO

Journal of Nematology, Volume 48 , ISSUE 3, 135–135

Research Article | 17-October-2018

First Report of Stubby-Root Nematode, Paratrichodorus minor, on Onion in Georgia, U.S.A

areas with healthy and infested plants, respectively. Because the male individuals were not found in the soil samples, females were used for species identification. Morphological and molecular analyses of females (Fig. 2A-C) identified the species as Paratrichodorus minor (Colbran) Siddiqi; (Decraemer, 1995). Nematode body shape was “cigar-shaped” with dorsally curved “onchiostyle” stylet Females had an oval-shaped vagina, vulva a transverse slit, and lateral body pores were absent. The measurements

Abolfazl Hajihassani, Negin Hamidi, Bhabesh Dutta, Chris Tyson

Journal of Nematology, Volume 50 , ISSUE 3, 453–455

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